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Literature summary extracted from

  • Gholami-Shabani, M.; Akbarzadeh, A.; Norouzian, D.; Amini, A.; Gholami-Shabani, Z.; Imani, A.; Chiani, M.; Riazi, G.; Shams-Ghahfarokhi, M.; Razzaghi-Abyaneh, M.
    Antimicrobial activity and physical characterization of silver nanoparticles green synthesized using nitrate reductase from Fusarium oxysporum (2014), Appl. Biochem. Biotechnol., 172, 4084-4098 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.7.1.3 medicine the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview Fusarium oxysporum
1.7.1.3 synthesis the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview Fusarium oxysporum

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.3 additional information the purified native enzyme is successfully used for synthesis of silver nanoparticles in an NADPH-dependent manner using gelatin as a capping agent, analysis by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy, overview. 0.2 M Phosphate buffer, pH 7.2, 1 mM silver nitrate as the enzyme substrate, 0.1 mg gelatin as a capping agent, 1 mM 4-hydroxyquinoline as an electron carrier, 1 mM NADPH as enzyme cofactor, and 0.1 mg of purified fungal nitrate reductase are incubated at 25°C for 5 h. The stable nonaggregating nanoparticles are spherical in shape with an average size of 50 nm and a zeta potential of -34.3. The synthesized nanoparticles show a strong growth inhibitory antimicrobial activity against all tested human pathogenic fungi and bacteria, overview Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.3 214000
-
 gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.3 nitrate + NADPH + H+ Fusarium oxysporum
-
 nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH + H+ Fusarium oxysporum IRAN 31C
-
 nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Fusarium oxysporum
-
-
-
1.7.1.3 Fusarium oxysporum IRAN 31C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.3 native enzyme 70fold by ultrafiltration, anion exchange chromatography, and gel filtration Fusarium oxysporum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.3 mycelium
-
 Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 177.1
-
 purified native enzyme, substrate nitrate, pH 7.2, 37°C Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH + H+
-
 Fusarium oxysporum nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + NADPH + H+
-
 Fusarium oxysporum IRAN 31C nitrite + NADP+ + H2O
-
 ?
1.7.1.3 nitrate + reduced methyl viologen
-
 Fusarium oxysporum nitrite + oxidized methyl viologen
-
 ?
1.7.1.3 nitrate + reduced methyl viologen
-
 Fusarium oxysporum IRAN 31C nitrite + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 heterotrimer 1 * 125000 + 1 * 60000 + 1 * 25000, SDS-PAGE Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.3 45
-
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.3 20 60 purified native nitrate reductase, pH 7.2, maintaining 50% of its original activity Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.3 7.2
-
 assay at Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.3 5 9 purified native nitrate reductase, maintaining 50% of its original activity Fusarium oxysporum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 NADPH
-
 Fusarium oxysporum