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Literature summary extracted from

  • Maalej, I.; Belhaj, I.; Masmoudi, N.F.; Belghith, H.
    Highly thermostable xylanase of the thermophilic fungus Talaromyces thermophilus purification and characterization (2009), Appl. Biochem. Biotechnol., 158, 200-212 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ba2+ 10 mM, 65% residual activity Thermomyces dupontii
3.2.1.8 dithiothreitol 10 mM, 85% residual activity Thermomyces dupontii
3.2.1.8 Hg2+ 10 mM, 65% residual activity Thermomyces dupontii
3.2.1.8 Mn2+ 10 mM, 65% residual activity Thermomyces dupontii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 birchwood xylan Km value 22.5 mg/ml, pH 7.0, 50°C Thermomyces dupontii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Thermomyces dupontii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Ag+ 10 mM, 125% of initial activity Thermomyces dupontii
3.2.1.8 Co2+ 10 mM, 112% of initial activity Thermomyces dupontii
3.2.1.8 Cu2+ 10 mM, 105% of initial activity Thermomyces dupontii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 25000
-
 gel filtration Thermomyces dupontii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermomyces dupontii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Thermomyces dupontii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 160
-
 pH 7.0, 50°C Thermomyces dupontii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Thermomyces dupontii ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Thermomyces dupontii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 25000, SDS-PAGE Thermomyces dupontii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
-
 Thermomyces dupontii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 stable for 7 days Thermomyces dupontii
3.2.1.8 100
-
 half-life 60 min Thermomyces dupontii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7.5
-
-
 Thermomyces dupontii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5
-
 80% of maximum activity Thermomyces dupontii
3.2.1.8 9
-
 85% of maximum activity Thermomyces dupontii
3.2.1.8 10
-
 60% of maximum activity Thermomyces dupontii

General Information

EC Number General Information Comment Organism
3.2.1.8 physiological function highest titer of xylanase (5.5 IU/ml) is obtained b y growth on oat spelt xylan Thermomyces dupontii