Literature summary extracted from

Rey, M.W.; Brown, K.M.; Golightly, E.J.; Fuglsang, C.C.; Nielsen, B.R.; Hendriksen, H.V.; Butterworth, A.; Xu, F.
Cloning, heterologous expression, and characterization of Thielavia terrestris glucoamylase (2003), Appl. Biochem. Biotechnol., 111, 153-166 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.3	expression in Aspergillus oryzae	Thermothielavioides terrestris

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.3	0.33	-	maltotriose	pH 4.0, 20°C	Thermothielavioides terrestris

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.2.1.3	extracellular	-	Thermothielavioides terrestris	-	-

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.3	Thermothielavioides terrestris	AAE85601	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.3	maltose + H2O	-	Thermothielavioides terrestris	2 D-glucose	-	?
3.2.1.3	maltotriose + H2O	-	Thermothielavioides terrestris	3 D-glucose	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.3	?	x * 75000, SDS-PAGE, recombinant protein, x * 62000, calculated	Thermothielavioides terrestris

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.3	GlaA	-	Thermothielavioides terrestris

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.2.1.3	60	-	-	Thermothielavioides terrestris

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.2.1.3	60	-	stable for 30 min	Thermothielavioides terrestris
3.2.1.3	70	-	30 min, 60-70% residual activity	Thermothielavioides terrestris

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2.1.3	91.7	-	maltotriose	pH 4.0, 20°C	Thermothielavioides terrestris

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.2.1.3	4	-	-	Thermothielavioides terrestris

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Release 2023.1 (January 2023)