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Literature summary extracted from

  • Jones, A.J.; Blaza, J.N.; Bridges, H.R.; May, B.; Moore, A.L.; Hirst, J.
    A self-assembled respiratory chain that catalyzes NADH oxidation by ubiquinone-10 cycling between complex I and the alternative oxidase (2016), Angew. Chem. Int. Ed. Engl., 55, 728-731 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
7.1.1.2 analysis kinetic analysis of enzyme using a seff-assembled proteoliposome system containing complex I, Q10, and quinol oxidase (the alternative oxidase, AOX) to recycle Q10H2 to Q10. Complex I is completely rate determining and the Q10 pool is kept oxidized under steady-state catalysis Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 additional information
-
Ubiquinone-10 KM value for complex I is 3.9 nmol/mg phospholipid Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion
-
Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.2 heart
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1.1.2 18.6
-
pH not specified in the publication, temperature not specified in the publication Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + ubiquinone-10 + 5 H+[side 1]
-
Bos taurus NAD+ + ubiquinol-10 + 4 H+[side 2]
-
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