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Literature summary extracted from

  • Rebelo, T.S.; Pereira, C.M.; Sales, M.G.; Noronha, J.P.; Costa-Rodrigues, J.; Silva, F.; Fernandes, M.H.
    Sarcosine oxidase composite screen-printed electrode for sarcosine determination in biological samples (2014), Anal. Chim. Acta, 850, 26-32 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.3.1 diagnostics developement of a simple and low cost electrochemical enzymatic biosensor for the determination of sarcosine in urine, based on the covalent immobilization of sarcosine oxidase, using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, on the surface of the screen-printed carbon electrode.The biosensor presents high analytical performance features, such as large concentration linear range (10-100 nM), low detection limit (16 nM) and large storage stability (60 days). The biosensor is successfully applied to the analysis of sarcosine in synthetic urine samples Bacillus sp. BSD-8
1.5.3.1 medicine developement of a simple and low cost electrochemical enzymatic biosensor for the determination of sarcosine in urine, based on the covalent immobilization of sarcosine oxidase, using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, on the surface of the screen-printed carbon electrode.The biosensor presents high analytical performance features, such as large concentration linear range (10-100 nM), low detection limit (16 nM) and large storage stability (60 days). The biosensor is successfully applied to the analysis of sarcosine in synthetic urine samples Bacillus sp. BSD-8
1.5.3.1 medicine the enzyme can be used as biosensor for the analysis of sarcosine in urine samples Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + H2O2 + O2 Bacillus sp. (in: Bacteria)
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 glycine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. (in: Bacteria)
-
-
-
1.5.3.1 Bacillus sp. BSD-8 Q6ITC6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2
-
 Bacillus sp. BSD-8 glycine + formaldehyde + H2O2
-
 ?
1.5.3.1 sarcosine + H2O2 + O2
-
 Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.1 SOX
-
 Bacillus sp. (in: Bacteria)