Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Weiss, M.S.; Pavlidis, I.V.; Vickers, C.; Hoehne, M.; Bornscheuer, U.T.
    Glycine oxidase based high-throughput solid-phase assay for substrate profiling and directed evolution of (R)- and (S)-selective amine transaminases (2014), Anal. Chem., 86, 11847-11853 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.19 expressed in Escherichia coli BL21(DE3) cells Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O Geobacillus kaustophilus
-
 glyoxylate + NH3
-
 ?
1.4.3.19 glycine + H2O + O2 Geobacillus kaustophilus
-
 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + O2 Geobacillus kaustophilus
-
 2-iminoacetate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Geobacillus kaustophilus Q5L2C2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.19 His-Trap column chromatography, and Superdex 200 gel filtration Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O
-
 Geobacillus kaustophilus glyoxylate + NH3
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Geobacillus kaustophilus glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + O2
-
 Geobacillus kaustophilus 2-iminoacetate + H2O2
-
 ?