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Literature summary extracted from

  • Jorge, J.M.; Leggewie, C.; Wendisch, V.F.
    A new metabolic route for the production of gamma-aminobutyric acid by Corynebacterium glutamicum from glucose (2016), Amino Acids, 48, 2519-2531 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.19 synthesis the enzyme is useful for GABA production in an engineered strain of Corynebacterium glutamicum produces 5.3 g/l of GABA Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.19 gene patD, recombinant expression in Corynebacterium glutamicum strain ATCC 13032 with deleted gabTDP operon and cgmA gene Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.19 additional information a putrescine-producing recombinant Corynebacterium glutamicum strain is converted into a 4-aminobutyric acid, GABA, producing strain by heterologous expression of putrescine transaminase (PatA) and gamma-aminobutyraldehyde dehydrogenase (PatD) genes from Escherichia coli. The resultant strain produces 5.3 g/l of GABA. GABA production is improved further by adjusting the concentration of nitrogen in the culture medium, by avoiding the formation of the by-product N-acetylputrescine and by deletion of the genes for GABA catabolism and GABA re-uptake Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.19 additional information addition of 4-aminobutyric acid, GABA, to the growth medium hardly affects growth of Corynebacterium glutamicum, since a half-inhibitory concentration of 1.1 M GABA is determined Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O Escherichia coli
-
 4-aminobutanoate + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Escherichia coli
-
 MG1655
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
 Escherichia coli 4-aminobutanoate + NADH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.19 gamma-aminobutyraldehyde dehydrogenase
-
 Escherichia coli
1.2.1.19 PatD
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.19 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 9.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.2.1.19 metabolism as alternative to GABA production by glutamate decarboxylation, another route for the production of GABA via putrescine is established in Corynebacterium glutamicum Escherichia coli