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Literature summary extracted from

  • Hayashida, S.; Yoshioka, H.
    Production and purification of thermostable cellulases from Humicola insolens YH-8 (1980), Agric. Biol. Chem., 44, 1721-1728 .
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Humicola insolens
-
-
-
3.2.1.4 Humicola insolens YH-8
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 17.3
-
 avicelase activity, pH 5.5, 50°C Humicola insolens
3.2.1.4 39.2
-
 carboxymethylcellulase activity, pH 5.5, 50°C Humicola insolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Humicola insolens D-glucose + ?
-
 ?
3.2.1.4 avicel + H2O
-
 Humicola insolens YH-8 D-glucose + ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Humicola insolens D-glucose + ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Humicola insolens YH-8 D-glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
 both carboxymethycellulase and avicelase acitivity Humicola insolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 carboxymethycellulase and avicelase activity, stable for 24 h Humicola insolens
3.2.1.4 65
-
 avicelase activity, stable for 5 min Humicola insolens
3.2.1.4 70
-
 carboxymethylcellulase activity, 5 min, 95% residual activity Humicola insolens
3.2.1.4 75
-
 avicelase activity, 20% residual activity Humicola insolens
3.2.1.4 95
-
 carboxymethylcellulase activity, 5 min, 45% residual activity Humicola insolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
 carboxymethycellulase activity Humicola insolens
3.2.1.4 5.3
-
 avicelase activity Humicola insolens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3.5 9.5 carboxymethycellulase and avicelase acitivity Humicola insolens

Expression

EC Number Organism Comment Expression
3.2.1.4 Humicola insolens highest expression at 50°C when cultivated on wheat bran up