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Literature summary extracted from

  • Vila, M.; Umpierrez, D.; Veiga, N.; Seoane, G.; Carrera, I.; Rodriguez Giordano, S.
    Site-directed mutagenesis studies on the toluene dioxygenase enzymatic system Role of phenylalanine 366, threonine 365 and isoleucine 324 in the chemo-, regio-, and stereoselectivity (2017), Adv. Synth. Catal., 359, 2149-2157 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 expressed in Escherichia coli JM109 cells Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.11 F366 the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas putida
1.14.12.11 I324F the mutant shows reduced activity compared to the wild type enzyme Pseudomonas putida
1.14.12.11 T365N the mutant shows slightly reduced activity compared to the wild type enzyme Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 1,3-dinitrobenzene + NADH + H+ + O2
-
 Pseudomonas putida ?
-
 ?
1.14.12.11 bromobenzene + NADH + H+ + O2
-
 Pseudomonas putida cis-(1S,2S)-3-bromocyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 indene + NADH + H+ + O2
-
 Pseudomonas putida cis-(1S,2R)-indandiol + (1S)-indenol + NAD+
-
 ?
1.14.12.11 propylbenzene + NADH + H+ + O2
-
 Pseudomonas putida cis-(1S,2R)-3-propylcyclohexa-3,5-diene-1,2-diol + cis-(1S,2R)-3-[(1R)-1-hydroxypropyl]cyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 styrene + NADH + H+ + O2
-
 Pseudomonas putida cis-2,3-dihydroxy-1-vinylcyclohexa-4,6-diene + (R)1-phenyl-1,2-ethanediol + NAD+
-
 ?
1.14.12.11 toluene + NADH + H+ + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.11 TDO
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida