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Literature summary extracted from
- Crystallization and preliminary X-ray crystallographic studies of dehydroascorbate reductase (DHAR) from Oryza sativa L. japonica (2014), Acta Crystallogr. Sect. F, 70, 781-785 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | expressed in Escherichia coli NiCo21 (DE3) cells | Oryza sativa Japonica Group |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | sitting drop vapor diffusion method, using 0.15 M potassium bromide, 30% (w/v) PEG MME 2000 | Oryza sativa Japonica Group |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.5.1 | cytosol | - |
Oryza sativa Japonica Group | 5829 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | Oryza sativa Japonica Group | - |
glutathione disulfide + ascorbate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | Oryza sativa Japonica Group | Q65XA0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | Ni-NTA resin column chromatography | Oryza sativa Japonica Group |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | - |
Oryza sativa Japonica Group | glutathione disulfide + ascorbate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | ? | x * 24000, SDS-PAGE | Oryza sativa Japonica Group |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | dehydroascorbate reductase | - |
Oryza sativa Japonica Group |
| 1.8.5.1 | DHAR | - |
Oryza sativa Japonica Group |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.8.5.1 | Oryza sativa Japonica Group | calculated from amino acid sequence | - |
5.8 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | physiological function | the increased level of antioxidants generated by the enzyme protects rice from oxidative damage and increases the yield of rice grains | Oryza sativa Japonica Group |
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