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Literature summary extracted from

  • Xu, Y.; Lu, Y.; Zhang, R.; Wang, H.; Liu, Q.
    Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes (2016), Acta Biochim. Pol., 63, 273-279 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 degradation laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Sarcomyxa serotina
1.10.3.2 environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Sarcomyxa serotina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol 12.5 mM, complete inhibition Sarcomyxa serotina
1.10.3.2 Al3+ 10 mM, 43% loss of activity Sarcomyxa serotina
1.10.3.2 Ca2+ 10 mM, 35% loss of activity Sarcomyxa serotina
1.10.3.2 Fe2+ 10 mM, 72.4% loss of activity Sarcomyxa serotina
1.10.3.2 K+ 10 mM, 7% loss of activity Sarcomyxa serotina
1.10.3.2 L-cysteine 50 mM, 15% inhibition Sarcomyxa serotina
1.10.3.2 Mg2+ 10 mM, 38% loss of activity Sarcomyxa serotina
1.10.3.2 Mn2+ 10 mM, 38% loss of activity Sarcomyxa serotina
1.10.3.2 additional information EDTA and DMSO have almost no inhibitory effect Sarcomyxa serotina
1.10.3.2 SDS 3 mM, 85% inhibition Sarcomyxa serotina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Zn2+ significantly enhances activity at concentrations under 50 mM Sarcomyxa serotina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 57000
-
 gel filtration Sarcomyxa serotina

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Sarcomyxa serotina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Sarcomyxa serotina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Sarcomyxa serotina ?
-
 ?
1.10.3.2 2-methylcatechol + O2 17.2% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina ?
-
 ?
1.10.3.2 N,N-dimethyl-1,4-phenylenediamine + O2 42.6% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina ?
-
 ?
1.10.3.2 pyrogallol + O2 7.7% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 57000, SDS-PAGE Sarcomyxa serotina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 30
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 20 50 20°C: about 40% of maximal activity, 50°C: about 60% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 3 h, at temperatures below 40°C, there is almost no change in enzymatic activity Sarcomyxa serotina
1.10.3.2 50
-
 3 h, at temperatures over 50°C the remaining laccase activity drops to below 70% of the initial activity Sarcomyxa serotina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6.8
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4 9 pH 4.0: about 65% of maximal activity, pH 9.0: about 70% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sarcomyxa serotina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 5.8
-
 2 h, 30% loss of activity Sarcomyxa serotina
1.10.3.2 6.2
-
 2 h, 27% loss of activity Sarcomyxa serotina
1.10.3.2 6.5
-
 2 h, 18% loss of activity Sarcomyxa serotina