EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.1.20 | K66Q/S149G/N262C | the mutant shows activity with phenylacetone and 4-phenyl-2-butanone | Rhodococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.20 | 1.4 | - |
4-phenyl-2-butanone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. | |
1.4.1.20 | 4 | - |
phenylacetone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.20 | L-phenylalanine + H2O + NAD+ | Rhodococcus sp. | - |
phenylpyruvate + NH3 + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.20 | Rhodococcus sp. | Q59771 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.20 | 4-phenyl-2-butanone + NH3 + NADH + H+ | substrate for mutant enzyme K66Q/S149G/N262C | Rhodococcus sp. | (R)-1-methyl-3-phenylpropylamine + H2O + NAD+ | - |
? | |
1.4.1.20 | L-phenylalanine + H2O + NAD+ | - |
Rhodococcus sp. | phenylpyruvate + NH3 + NADH + H+ | - |
? | |
1.4.1.20 | phenylacetone + NH3 + NADH + H+ | substrate for mutant enzyme K66Q/S149G/N262C | Rhodococcus sp. | (R)-amphetamine + H2O + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.20 | PheDH | - |
Rhodococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.20 | 0.7 | - |
phenylacetone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. | |
1.4.1.20 | 0.72 | - |
4-phenyl-2-butanone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.20 | NAD+ | - |
Rhodococcus sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.20 | 0.18 | - |
phenylacetone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. | |
1.4.1.20 | 0.5 | - |
4-phenyl-2-butanone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. |