BRENDA - Enzyme Database

Structural basis for the catalytic mechanism of DncV, bacterial homolog of cyclic GMP-AMP synthase

Kato, K.; Ishii, R.; Hirano, S.; Ishitani, R.; Nureki, O.; Structure 23, 843-850 (2015)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
2.7.7.B24
structure in complex with dGTP. DncV adopts an extended bilobed architecture characterized by a nucleotidyltransferase fold
Vibrio cholerae O1
2.7.7.B24
structure of apo form and in complex with GTP and with dATP and dGTP, respectively. DncV adopts an extended bilobed architecture characterized by a nucleotidyltransferase fold. The ribose and triphosphate moieties of both GTP and ATP are bound to the donor and acceptor pockets in a similar manner. DncV efficiently catalyzes the phosphodiester linkage formation with ATP bound to the acceptor pocket, compared with GTP, thereby producing pppA(3'–5')pG preferentially
Vibrio cholerae O1
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.7.B24
Vibrio cholerae O1
Q6XGD8
-
-
2.7.7.B24
Vibrio cholerae O1
Q9KVG7
-
-
2.7.7.B24
Vibrio cholerae O1 ATCC 39315
Q6XGD8
-
-
2.7.7.B24
Vibrio cholerae O1 ATCC 39315
Q9KVG7
-
-
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
2.7.7.B24
ATP + GTP = 2 diphosphate + cyclic Gp(3'-5')G
(3)
Vibrio cholerae O1
2.7.7.B24
GTP + ATP = 2 diphosphate + cyclic Gp(3'-5')A
(2)
Vibrio cholerae O1
2.7.7.B24
GTP + GTP = 2 diphosphate + cyclic Gp(3'-5')G
(1)
Vibrio cholerae O1
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.7.B24
ATP + GTP
-
741448
Vibrio cholerae O1
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
ATP + GTP
-
741448
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
GTP + ATP
-
741448
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')A
-
-
-
?
2.7.7.B24
GTP + ATP
-
741448
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')A
-
-
-
?
2.7.7.B24
GTP + GTP
-
741448
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
2.7.7.B24
GTP + GTP
-
741448
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
2.7.7.B24
additional information
DncV preferably recognizes ATP and GTP as acceptor and donor nucleotides, respectively, in the first nucleotidyl transfer reaction
741448
Vibrio cholerae O1
?
-
-
-
?
2.7.7.B24
additional information
DncV preferably recognizes ATP and GTP as acceptor and donor nucleotides, respectively, in the first nucleotidyl transfer reaction
741448
Vibrio cholerae O1 ATCC 39315
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.7.B24
3'-5'-cyclic AMP GMP cyclase
-
Vibrio cholerae O1
2.7.7.B24
DncV
-
Vibrio cholerae O1
2.7.7.B24
VC0179-like protein
-
Vibrio cholerae O1
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.7.7.B24
structure in complex with dGTP. DncV adopts an extended bilobed architecture characterized by a nucleotidyltransferase fold
Vibrio cholerae O1
2.7.7.B24
structure of apo form and in complex with GTP and with dATP and dGTP, respectively. DncV adopts an extended bilobed architecture characterized by a nucleotidyltransferase fold. The ribose and triphosphate moieties of both GTP and ATP are bound to the donor and acceptor pockets in a similar manner. DncV efficiently catalyzes the phosphodiester linkage formation with ATP bound to the acceptor pocket, compared with GTP, thereby producing pppA(3'–5')pG preferentially
Vibrio cholerae O1
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.7.B24
ATP + GTP
-
741448
Vibrio cholerae O1
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
ATP + GTP
-
741448
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Ap(3'-5')G
-
-
-
?
2.7.7.B24
GTP + ATP
-
741448
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')A
-
-
-
?
2.7.7.B24
GTP + ATP
-
741448
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')A
-
-
-
?
2.7.7.B24
GTP + GTP
-
741448
Vibrio cholerae O1
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
2.7.7.B24
GTP + GTP
-
741448
Vibrio cholerae O1 ATCC 39315
2 diphosphate + cyclic Gp(3'-5')G
-
-
-
?
2.7.7.B24
additional information
DncV preferably recognizes ATP and GTP as acceptor and donor nucleotides, respectively, in the first nucleotidyl transfer reaction
741448
Vibrio cholerae O1
?
-
-
-
?
2.7.7.B24
additional information
DncV preferably recognizes ATP and GTP as acceptor and donor nucleotides, respectively, in the first nucleotidyl transfer reaction
741448
Vibrio cholerae O1 ATCC 39315
?
-
-
-
?