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Literature summary extracted from

  • Kato, K.; Ishii, R.; Hirano, S.; Ishitani, R.; Nureki, O.
    Structural basis for the catalytic mechanism of DncV, bacterial homolog of cyclic GMP-AMP synthase (2015), Structure, 23, 843-850.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.B24 structure in complex with dGTP. DncV adopts an extended bilobed architecture characterized by a nucleotidyltransferase fold Vibrio cholerae O1
2.7.7.B24 structure of apo form and in complex with GTP and with dATP and dGTP, respectively. DncV adopts an extended bilobed architecture characterized by a nucleotidyltransferase fold. The ribose and triphosphate moieties of both GTP and ATP are bound to the donor and acceptor pockets in a similar manner. DncV efficiently catalyzes the phosphodiester linkage formation with ATP bound to the acceptor pocket, compared with GTP, thereby producing pppA(3'–5')pG preferentially Vibrio cholerae O1

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B24 Vibrio cholerae O1 Q6XGD8
-
-
2.7.7.B24 Vibrio cholerae O1 Q9KVG7
-
-
2.7.7.B24 Vibrio cholerae O1 ATCC 39315 Q6XGD8
-
-
2.7.7.B24 Vibrio cholerae O1 ATCC 39315 Q9KVG7
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.B24 ATP + GTP = 2 diphosphate + cyclic Gp(3'-5')G (3) Vibrio cholerae O1
a
2.7.7.B24 GTP + ATP = 2 diphosphate + cyclic Gp(3'-5')A (2) Vibrio cholerae O1
a
2.7.7.B24 GTP + GTP = 2 diphosphate + cyclic Gp(3'-5')G (1) Vibrio cholerae O1
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B24 ATP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
2.7.7.B24 ATP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
2.7.7.B24 GTP + ATP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Gp(3'-5')A
-
 ?
2.7.7.B24 GTP + ATP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Gp(3'-5')A
-
 ?
2.7.7.B24 GTP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Gp(3'-5')G
-
 ?
2.7.7.B24 GTP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Gp(3'-5')G
-
 ?
2.7.7.B24 additional information DncV preferably recognizes ATP and GTP as acceptor and donor nucleotides, respectively, in the first nucleotidyl transfer reaction Vibrio cholerae O1 ?
-
 ?
2.7.7.B24 additional information DncV preferably recognizes ATP and GTP as acceptor and donor nucleotides, respectively, in the first nucleotidyl transfer reaction Vibrio cholerae O1 ATCC 39315 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B24 3'-5'-cyclic AMP GMP cyclase
-
 Vibrio cholerae O1
2.7.7.B24 DncV
-
 Vibrio cholerae O1
2.7.7.B24 VC0179-like protein
-
 Vibrio cholerae O1