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Literature summary extracted from

  • Vuong, T.V.; Foumani, M.; MacCormick, B.; Kwan, R.; Master, E.R.
    Direct comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability (2016), Sci. Rep., 6, 37356.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.B3 expressed in Pichia pastoris KM71H Sarocladium strictum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.B3 Y300A the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme Sarocladium strictum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.B3 H2O2 about 40% inhibition at 200 mM Sarocladium strictum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.B3 0.25
-
cellobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 0.25
-
cellotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 3.15
-
xylotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 5.11
-
xylobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 8.1
-
D-glucose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 51.8
-
D-xylose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B3 Sarocladium strictum D7PF15
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.B3 Ni-NTA resin column chromatography Sarocladium strictum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B3 arabinoxylan + O2
-
Sarocladium strictum ?
-
?
1.1.99.B3 beech wood xylan + O2
-
Sarocladium strictum ?
-
?
1.1.99.B3 cellobiose + O2
-
Sarocladium strictum cellobiono-1,5-lactone + H2O2
-
?
1.1.99.B3 cellotriose + O2
-
Sarocladium strictum cellotriono-1,5-lactone + H2O2
-
?
1.1.99.B3 D-glucose + O2
-
Sarocladium strictum D-glucono-1,5-lactone + H2O2
-
?
1.1.99.B3 D-xylose + O2
-
Sarocladium strictum D-xylono-1,5-lactone + H2O2
-
?
1.1.99.B3 maltose + O2
-
Sarocladium strictum maltono-1,5-lactone + H2O2
-
?
1.1.99.B3 propoxylated wheat bran hemicellulose + O2
-
Sarocladium strictum ?
-
?
1.1.99.B3 wheat bran hemicellulose + O2
-
Sarocladium strictum ?
-
?
1.1.99.B3 xylobiose + O2 highest activity Sarocladium strictum xylobiono-1,5-lactone + H2O2
-
?
1.1.99.B3 xylotriose + O2
-
Sarocladium strictum xylotriono-1,5-lactone + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.B3 gluco-oligosaccharide oxidase
-
Sarocladium strictum
1.1.99.B3 GOOX
-
Sarocladium strictum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.B3 11.2
-
cellotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 11.3
-
D-xylose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 13.2
-
D-glucose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 13.3
-
xylobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 13.7
-
cellobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 13.9
-
xylotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.B3 0.22
-
D-xylose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 1.6
-
D-glucose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 2.6
-
xylobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 4.3
-
xylotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 45
-
cellotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.1.99.B3 56.7
-
cellobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum