EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.B3 | expressed in Pichia pastoris KM71H | Sarocladium strictum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.99.B3 | Y300A | the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme | Sarocladium strictum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.B3 | H2O2 | about 40% inhibition at 200 mM | Sarocladium strictum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.B3 | 0.25 | - |
cellobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 0.25 | - |
cellotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 3.15 | - |
xylotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 5.11 | - |
xylobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 8.1 | - |
D-glucose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 51.8 | - |
D-xylose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.B3 | Sarocladium strictum | D7PF15 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.B3 | Ni-NTA resin column chromatography | Sarocladium strictum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.B3 | arabinoxylan + O2 | - |
Sarocladium strictum | ? | - |
? | |
1.1.99.B3 | beech wood xylan + O2 | - |
Sarocladium strictum | ? | - |
? | |
1.1.99.B3 | cellobiose + O2 | - |
Sarocladium strictum | cellobiono-1,5-lactone + H2O2 | - |
? | |
1.1.99.B3 | cellotriose + O2 | - |
Sarocladium strictum | cellotriono-1,5-lactone + H2O2 | - |
? | |
1.1.99.B3 | D-glucose + O2 | - |
Sarocladium strictum | D-glucono-1,5-lactone + H2O2 | - |
? | |
1.1.99.B3 | D-xylose + O2 | - |
Sarocladium strictum | D-xylono-1,5-lactone + H2O2 | - |
? | |
1.1.99.B3 | maltose + O2 | - |
Sarocladium strictum | maltono-1,5-lactone + H2O2 | - |
? | |
1.1.99.B3 | propoxylated wheat bran hemicellulose + O2 | - |
Sarocladium strictum | ? | - |
? | |
1.1.99.B3 | wheat bran hemicellulose + O2 | - |
Sarocladium strictum | ? | - |
? | |
1.1.99.B3 | xylobiose + O2 | highest activity | Sarocladium strictum | xylobiono-1,5-lactone + H2O2 | - |
? | |
1.1.99.B3 | xylotriose + O2 | - |
Sarocladium strictum | xylotriono-1,5-lactone + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.B3 | gluco-oligosaccharide oxidase | - |
Sarocladium strictum |
1.1.99.B3 | GOOX | - |
Sarocladium strictum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.B3 | 11.2 | - |
cellotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 11.3 | - |
D-xylose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 13.2 | - |
D-glucose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 13.3 | - |
xylobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 13.7 | - |
cellobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 13.9 | - |
xylotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.B3 | 0.22 | - |
D-xylose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 1.6 | - |
D-glucose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 2.6 | - |
xylobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 4.3 | - |
xylotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 45 | - |
cellotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.1.99.B3 | 56.7 | - |
cellobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum |