EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.44 | expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
1.1.1.343 | expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.44 | N32D | the mutant exhibits an 460times higher Km value on NADP+ and a slightly decreased Km value of 4.0 mM on NAD+ compared with the wild type enzyme | Thermotoga maritima |
1.1.1.44 | N32D/R33I/T34I | the mutant exhibits a far higher Km value of on NADP+ and a slightly decreased Km value on NAD+ compared with the wild type enzyme | Thermotoga maritima |
1.1.1.44 | N32D/R33L/T34S | the mutant has an increased Km value on NADP+ and NAD+ compared to the wild type enzyme | Thermotoga maritima |
1.1.1.44 | N32E/R33I/T34I | the mutant exhibits an about 6400fold reversal of the coenzyme selectivity from NADP+ to NAD+ compared to the wild type enzyme | Thermotoga maritima |
1.1.1.343 | N32D | the mutant exhibits an about 1.3fold increase of catalytic efficiency with NAD+ as cofactor compared to the wild type enzyme | Thermotoga maritima |
1.1.1.343 | N32D/R33I/T34I | the mutant exhibits an about 1.5fold increase of catalytic efficiency with NAD+ as cofactor compared to the wild type enzyme | Thermotoga maritima |
1.1.1.343 | N32D/R33L/T34S | the mutant exhibits an about 0.6fold decrease of catalytic efficiency with NAD+ as cofactor compared to the wild type enzyme | Thermotoga maritima |
1.1.1.343 | N32E/R33I/T34I | the mutant exhibits an about 3.5fold increase of catalytic efficiency with NAD+ as cofactor, with about 64000fold reversal of the coenzyme selectivity from NADP+ to NAD+ compared to the wild type enzyme | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.44 | 0.005 | - |
NADP+ | wild type enzyme, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 2.3 | - |
NADP+ | mutant enzyme N32D, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 2.5 | - |
NAD+ | mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 3.9 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 4 | - |
NAD+ | mutant enzyme N32D, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 4.3 | - |
NAD+ | wild type enzyme at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 7.5 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 70 | - |
NADP+ | mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 79 | - |
NADP+ | mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 100 | - |
NADP+ | Km above 100 mM, mutant enzyme N32D/R33L/T34S, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.343 | 2.5 | - |
NAD+ | mutant enzyme N32E/R33I7T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 3.9 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 4 | - |
NAD+ | mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 4.3 | - |
NAD+ | wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 7.5 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Thermotoga maritima | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? | |
1.1.1.343 | 6-phospho-D-gluconate + NAD+ | Thermotoga maritima | - |
D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.44 | Thermotoga maritima | Q9WYR9 | - |
- |
1.1.1.343 | Thermotoga maritima | Q9WYR9 | cf EC 1.1.1.44 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.44 | HisPur Ni-NTA resin column chromatography | Thermotoga maritima |
1.1.1.343 | Ni-NTA resin column chromatography | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.44 | 6-phospho-D-gluconate + NAD+ | low activity with NAD+ | Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? | |
1.1.1.343 | 6-phospho-D-gluconate + NAD+ | - |
Thermotoga maritima | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.44 | 6-phosphogluconate dehydrogenase | - |
Thermotoga maritima |
1.1.1.44 | 6PGDH | - |
Thermotoga maritima |
1.1.1.343 | 6PGDH | - |
Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.44 | 15.9 | - |
NADP+ | mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 15.9 | - |
NADP+ | mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 16.7 | - |
NADP+ | mutant enzyme N32D, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 17.6 | - |
NADP+ | wild type enzyme, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 23.3 | - |
NAD+ | wild type enzyme at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 25.4 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 29.3 | - |
NAD+ | mutant enzyme N32D, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 32.2 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 47.9 | - |
NAD+ | mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.343 | 23.3 | - |
NAD+ | wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 25.4 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 29.3 | - |
NAD+ | mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 32.2 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 47.9 | - |
NAD+ | mutant enzyme N32E/R33I7T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.44 | NAD+ | low activity with NAD+ | Thermotoga maritima | |
1.1.1.44 | NADP+ | natural cofactor | Thermotoga maritima | |
1.1.1.343 | NAD+ | - |
Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.44 | 0.2 | - |
NADP+ | mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 0.2 | - |
NADP+ | mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 3.4 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 5.4 | - |
NAD+ | wild type enzyme at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 7.3 | - |
NAD+ | mutant enzyme N32D, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 7.3 | - |
NADP+ | mutant enzyme N32D, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 8.3 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 19.2 | - |
NAD+ | mutant enzyme N32E/R33I/T34I, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.44 | 3520 | - |
NADP+ | wild type enzyme, at pH 7.5 and 70°C | Thermotoga maritima | |
1.1.1.343 | 3.4 | - |
NAD+ | mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 5.4 | - |
NAD+ | wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 7.3 | - |
NAD+ | mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 8.3 | - |
NAD+ | mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima | |
1.1.1.343 | 19.2 | - |
NAD+ | mutant enzyme N32E/R33I7T34I, in 100 mM HEPES (pH 7.5), at 70°C | Thermotoga maritima |