BRENDA - Enzyme Database

Novel type II and monomeric NAD+ specific isocitrate dehydrogenases: phylogenetic affinity, enzymatic characterization, and evolutionary implication

Wang, P.; Lv, C.; Zhu, G.; Sci. Rep. 5, 9150 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.41
gene HMPREF1139_0890, sequence comparisons and phylogenetic analysis, recombinant overexpression of wild-type and mutant His6-tagged enzymes in Escherichia coli
Campylobacter sp.
1.1.1.41
gene icd, sequence comparisons and phylogenetic analysis
Campylobacter curvus
1.1.1.41
gene icd, sequence comparisons and phylogenetic analysis, recombinant overexpression of wild-type and mutant His6-tagged enzymes in Escherichia coli
Ostreococcus sp. 'lucimarinus'
1.1.1.41
gene IDHP, sequence comparisons and phylogenetic analysis
Micromonas commoda
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
D326R/M327H
site-directed mutagenesis, the coenzyme specificity of the mutant CaIDH Asp326/Met327 is completely reversed from NAD+ to NADP+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
D326X/M327X
site-directed mutagenesis, the coenzyme specificity of the mutant OlIDH R326H327 is completely reversed from NAD+ to NADP+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
L584H/D595R
site-directed mutagenesis, the coenzyme specificity of the mutant CaIDH H584/R595 is completely reversed from NAD+ to NADP+
Campylobacter sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
Ca2+
complete inhibition at 2 mM
Campylobacter sp.
1.1.1.41
Cu2+
complete inhibition at 2 mM
Campylobacter sp.
1.1.1.41
Cu2+
complete inhibition at 2 mM
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Zn2+
complete inhibition at 2 mM
Campylobacter sp.
1.1.1.41
Zn2+
complete inhibition at 2 mM
Ostreococcus sp. 'lucimarinus'
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.0082
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
0.0114
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
0.0289
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
0.0742
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
0.126
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
0.136
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
0.476
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
0.493
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
0.513
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
1.827
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
2.211
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
2.948
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Ca2+
activates, 13.6% activity compared to Mg2+ for OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Co2+
activates, 35.5% activity compared to Mg2+ for CaIDH
Campylobacter sp.
1.1.1.41
Mg2+
activates, 78.3% activity compared to Mg2+ for CaIDH
Campylobacter sp.
1.1.1.41
Mg2+
activates, 68.6% activity compared to Mg2+ for OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Mn2+
activates, Mn2+ is the most favorable cation for CaIDH
Campylobacter sp.
1.1.1.41
Mn2+
activates, Mn2+ is the most favorable cation for OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
additional information
divalent cations are required for activity
Campylobacter curvus
1.1.1.41
additional information
divalent cations are required for activity, low or poor activation by Rb+, Ni2+, K+, Na+, and Li+
Campylobacter sp.
1.1.1.41
additional information
divalent cations are required for activity
Micromonas commoda
1.1.1.41
additional information
divalent cations are required for activity, low or poor activation by Co2+, Ni2+, K+, Na+, and Li+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Rb+
activates, 11.0% activity compared to Mg2+ for OlIDH
Ostreococcus sp. 'lucimarinus'
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
45000
-
-
Ostreococcus sp. 'lucimarinus'
1.1.1.41
80000
-
-
Campylobacter sp.
1.1.1.41
81000
-
recombinant His6-tagged enzyme, gel filtration
Campylobacter sp.
1.1.1.41
92000
-
recombinant His6-tagged enzyme, gel filtration
Ostreococcus sp. 'lucimarinus'
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Ostreococcus sp. 'lucimarinus'
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Micromonas commoda
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter sp.
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter curvus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Ostreococcus sp. 'lucimarinus' CCE9901
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter curvus 525.92
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter sp. FOBRC14
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Micromonas commoda RCC299
-
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Campylobacter curvus
A0A0M4S9Q1
-
-
1.1.1.41
Campylobacter curvus 525.92
A0A0M4S9Q1
-
-
1.1.1.41
Campylobacter sp.
J4X756
-
-
1.1.1.41
Campylobacter sp. FOBRC14
J4X756
-
-
1.1.1.41
Micromonas commoda
C1E5X2
-
-
1.1.1.41
Micromonas commoda RCC299
C1E5X2
-
-
1.1.1.41
Ostreococcus sp. 'lucimarinus'
A4SBK5
-
-
1.1.1.41
Ostreococcus sp. 'lucimarinus'
-
-
-
1.1.1.41
Ostreococcus sp. 'lucimarinus' CCE9901
A4SBK5
-
-
1.1.1.41
Ostreococcus sp. 'lucimarinus' CCE9901
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli
Campylobacter sp.
1.1.1.41
recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli
Ostreococcus sp. 'lucimarinus'
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
3.8
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NADP+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
8.7
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NADP+
Campylobacter sp.
1.1.1.41
53.2
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NAD+
Campylobacter sp.
1.1.1.41
72.3
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NAD+
Ostreococcus sp. 'lucimarinus'
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
741414
Ostreococcus sp. 'lucimarinus'
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Micromonas commoda
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter sp.
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter curvus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Ostreococcus sp. 'lucimarinus' CCE9901
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter curvus 525.92
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter sp. FOBRC14
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Micromonas commoda RCC299
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Micromonas commoda
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter sp.
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter curvus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Ostreococcus sp. 'lucimarinus'
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Ostreococcus sp. 'lucimarinus' CCE9901
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter curvus 525.92
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter sp. FOBRC14
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Micromonas commoda RCC299
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
homodimer
-
Micromonas commoda
1.1.1.41
homodimer
; 2 * 45000, recombinant His6-tagged enzyme, SDS-PAGE
Ostreococcus sp. 'lucimarinus'
1.1.1.41
monomer
-
Campylobacter curvus
1.1.1.41
monomer
1 * 80000, recombinant His6-tagged enzyme, SDS-PAGE
Campylobacter sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
25
-
assay at
Campylobacter curvus
1.1.1.41
25
-
assay at
Micromonas commoda
1.1.1.41
40
-
with Mn2+
Campylobacter sp.
1.1.1.41
40
-
with Mg2+ or Mn2+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
45
-
with Mg2+
Campylobacter sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
40
55
recombinant CaIDH retains the majority of the activity below 45°C, its activity drops rapidly as the temperature is raised. Incubation at 55°C for 20 min causes a 55% or 75% loss of activity in the presence of Mg2+ or Mn2+, respectively
Campylobacter sp.
1.1.1.41
45
50
recombinant OlIDH is stable below 45°C, but its activity rapidly declines as the temperature is raised. Incubation at 45°C for 20 min causes a 28% or 21% loss of activity in the presence of Mg2+ or Mn2+, respectively, whereas incubation at 50°C causes a 91% or 84% loss of activity in the presence of Mg2+ or Mn2+, respectively
Ostreococcus sp. 'lucimarinus'
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.41
1.4
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
1.8
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
1.9
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
2
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
4.2
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
7
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
9.5
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
10
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
10.8
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
22.5
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
29.1
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
60.6
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
assay at
Campylobacter curvus
1.1.1.41
7.5
-
with Mn2+
Campylobacter sp.
1.1.1.41
7.5
-
assay at
Micromonas commoda
1.1.1.41
8
-
with Mg2+
Campylobacter sp.
1.1.1.41
8.5
-
with Mn2+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
9
-
with Mg2+
Ostreococcus sp. 'lucimarinus'
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
specific for, the enzyme displays 37fold preference for NAD+ over NADP+
Campylobacter curvus
1.1.1.41
NAD+
specific for, the enzyme displays 61fold preference for NAD+ over NADP+. Putative coenzyme discriminating amino acids residues are Leu584 and Asp595 in CaIDH
Campylobacter sp.
1.1.1.41
NAD+
specific for, the enzyme displays 224fold preference for NAD+ over NADP+
Micromonas commoda
1.1.1.41
NAD+
specific for, the enzyme displays 99fold preference for NAD+ over NADP+; specific for, the enzyme displays 99fold preference for NAD+ over NADP+. Putative coenzyme discriminating amino acids residues are Asp326 and Met327 in OlIDH
Ostreococcus sp. 'lucimarinus'
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
gene HMPREF1139_0890, sequence comparisons and phylogenetic analysis, recombinant overexpression of wild-type and mutant His6-tagged enzymes in Escherichia coli
Campylobacter sp.
1.1.1.41
gene icd, sequence comparisons and phylogenetic analysis
Campylobacter curvus
1.1.1.41
gene icd, sequence comparisons and phylogenetic analysis, recombinant overexpression of wild-type and mutant His6-tagged enzymes in Escherichia coli
Ostreococcus sp. 'lucimarinus'
1.1.1.41
gene IDHP, sequence comparisons and phylogenetic analysis
Micromonas commoda
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
specific for, the enzyme displays 37fold preference for NAD+ over NADP+
Campylobacter curvus
1.1.1.41
NAD+
specific for, the enzyme displays 61fold preference for NAD+ over NADP+. Putative coenzyme discriminating amino acids residues are Leu584 and Asp595 in CaIDH
Campylobacter sp.
1.1.1.41
NAD+
specific for, the enzyme displays 224fold preference for NAD+ over NADP+
Micromonas commoda
1.1.1.41
NAD+
specific for, the enzyme displays 99fold preference for NAD+ over NADP+. Putative coenzyme discriminating amino acids residues are Asp326 and Met327 in OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
NAD+
specific for, the enzyme displays 99fold preference for NAD+ over NADP+
Ostreococcus sp. 'lucimarinus'
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
D326R/M327H
site-directed mutagenesis, the coenzyme specificity of the mutant CaIDH Asp326/Met327 is completely reversed from NAD+ to NADP+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
D326X/M327X
site-directed mutagenesis, the coenzyme specificity of the mutant OlIDH R326H327 is completely reversed from NAD+ to NADP+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
L584H/D595R
site-directed mutagenesis, the coenzyme specificity of the mutant CaIDH H584/R595 is completely reversed from NAD+ to NADP+
Campylobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
Ca2+
complete inhibition at 2 mM
Campylobacter sp.
1.1.1.41
Cu2+
complete inhibition at 2 mM
Campylobacter sp.
1.1.1.41
Cu2+
complete inhibition at 2 mM
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Zn2+
complete inhibition at 2 mM
Campylobacter sp.
1.1.1.41
Zn2+
complete inhibition at 2 mM
Ostreococcus sp. 'lucimarinus'
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.0082
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
0.0114
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
0.0289
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
0.0742
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
0.126
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
0.136
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
0.476
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
0.493
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
0.513
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
1.827
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
2.211
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
2.948
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Ca2+
activates, 13.6% activity compared to Mg2+ for OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Co2+
activates, 35.5% activity compared to Mg2+ for CaIDH
Campylobacter sp.
1.1.1.41
Mg2+
activates, 78.3% activity compared to Mg2+ for CaIDH
Campylobacter sp.
1.1.1.41
Mg2+
activates, 68.6% activity compared to Mg2+ for OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Mn2+
activates, Mn2+ is the most favorable cation for CaIDH
Campylobacter sp.
1.1.1.41
Mn2+
activates, Mn2+ is the most favorable cation for OlIDH
Ostreococcus sp. 'lucimarinus'
1.1.1.41
additional information
divalent cations are required for activity
Campylobacter curvus
1.1.1.41
additional information
divalent cations are required for activity, low or poor activation by Rb+, Ni2+, K+, Na+, and Li+
Campylobacter sp.
1.1.1.41
additional information
divalent cations are required for activity
Micromonas commoda
1.1.1.41
additional information
divalent cations are required for activity, low or poor activation by Co2+, Ni2+, K+, Na+, and Li+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
Rb+
activates, 11.0% activity compared to Mg2+ for OlIDH
Ostreococcus sp. 'lucimarinus'
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
45000
-
-
Ostreococcus sp. 'lucimarinus'
1.1.1.41
80000
-
-
Campylobacter sp.
1.1.1.41
81000
-
recombinant His6-tagged enzyme, gel filtration
Campylobacter sp.
1.1.1.41
92000
-
recombinant His6-tagged enzyme, gel filtration
Ostreococcus sp. 'lucimarinus'
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Ostreococcus sp. 'lucimarinus'
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Micromonas commoda
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter sp.
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter curvus
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Ostreococcus sp. 'lucimarinus' CCE9901
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter curvus 525.92
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Campylobacter sp. FOBRC14
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Micromonas commoda RCC299
-
2-oxoglutarate + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli
Campylobacter sp.
1.1.1.41
recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli
Ostreococcus sp. 'lucimarinus'
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.41
3.8
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NADP+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
8.7
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NADP+
Campylobacter sp.
1.1.1.41
53.2
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NAD+
Campylobacter sp.
1.1.1.41
72.3
-
purified recombinant His6-tagged enzyme, pH 7.5, 25°C, with NAD+
Ostreococcus sp. 'lucimarinus'
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
741414
Ostreococcus sp. 'lucimarinus'
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Micromonas commoda
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter sp.
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter curvus
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Ostreococcus sp. 'lucimarinus' CCE9901
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter curvus 525.92
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Campylobacter sp. FOBRC14
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
741414
Micromonas commoda RCC299
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Micromonas commoda
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter sp.
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter curvus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Ostreococcus sp. 'lucimarinus'
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Ostreococcus sp. 'lucimarinus' CCE9901
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter curvus 525.92
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Campylobacter sp. FOBRC14
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
very low activity
741414
Micromonas commoda RCC299
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
homodimer
-
Micromonas commoda
1.1.1.41
homodimer
-
Ostreococcus sp. 'lucimarinus'
1.1.1.41
homodimer
2 * 45000, recombinant His6-tagged enzyme, SDS-PAGE
Ostreococcus sp. 'lucimarinus'
1.1.1.41
monomer
-
Campylobacter curvus
1.1.1.41
monomer
1 * 80000, recombinant His6-tagged enzyme, SDS-PAGE
Campylobacter sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
25
-
assay at
Campylobacter curvus
1.1.1.41
25
-
assay at
Micromonas commoda
1.1.1.41
40
-
with Mn2+
Campylobacter sp.
1.1.1.41
40
-
with Mg2+ or Mn2+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
45
-
with Mg2+
Campylobacter sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
40
55
recombinant CaIDH retains the majority of the activity below 45°C, its activity drops rapidly as the temperature is raised. Incubation at 55°C for 20 min causes a 55% or 75% loss of activity in the presence of Mg2+ or Mn2+, respectively
Campylobacter sp.
1.1.1.41
45
50
recombinant OlIDH is stable below 45°C, but its activity rapidly declines as the temperature is raised. Incubation at 45°C for 20 min causes a 28% or 21% loss of activity in the presence of Mg2+ or Mn2+, respectively, whereas incubation at 50°C causes a 91% or 84% loss of activity in the presence of Mg2+ or Mn2+, respectively
Ostreococcus sp. 'lucimarinus'
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.41
1.4
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
1.8
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
1.9
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
2
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
4.2
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
7
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
9.5
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
10
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
10.8
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
22.5
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
29.1
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
60.6
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
assay at
Campylobacter curvus
1.1.1.41
7.5
-
with Mn2+
Campylobacter sp.
1.1.1.41
7.5
-
assay at
Micromonas commoda
1.1.1.41
8
-
with Mg2+
Campylobacter sp.
1.1.1.41
8.5
-
with Mn2+
Ostreococcus sp. 'lucimarinus'
1.1.1.41
9
-
with Mg2+
Ostreococcus sp. 'lucimarinus'
General Information
EC Number
General Information
Commentary
Organism
1.1.1.41
evolution
evolutionary relationships of IDHs, overview. The enzyme belongs to the IDH enzyme family and the subclade of monomeric enzymes
Campylobacter curvus
1.1.1.41
evolution
evolutionary relationships of IDHs, overview. The enzyme belongs to the IDH enzyme family and the subclade of monomeric enzymes
Campylobacter sp.
1.1.1.41
evolution
evolutionary relationships of IDHs, phylogenetic analysis, overview. The enzyme belongs to the IDH enzyme family and the subclade of type II homodimeric enzymes
Micromonas commoda
1.1.1.41
evolution
evolutionary relationships of IDHs, overview; evolutionary relationships of IDHs, overview. The enzyme belongs to the IDH enzyme family and is clustered into a unique clade among the type II subfamily
Ostreococcus sp. 'lucimarinus'
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Campylobacter curvus
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Campylobacter sp.
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Micromonas commoda
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Ostreococcus sp. 'lucimarinus'
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.41
evolution
evolutionary relationships of IDHs, overview. The enzyme belongs to the IDH enzyme family and the subclade of monomeric enzymes
Campylobacter curvus
1.1.1.41
evolution
evolutionary relationships of IDHs, overview. The enzyme belongs to the IDH enzyme family and the subclade of monomeric enzymes
Campylobacter sp.
1.1.1.41
evolution
evolutionary relationships of IDHs, phylogenetic analysis, overview. The enzyme belongs to the IDH enzyme family and the subclade of type II homodimeric enzymes
Micromonas commoda
1.1.1.41
evolution
evolutionary relationships of IDHs, overview
Ostreococcus sp. 'lucimarinus'
1.1.1.41
evolution
evolutionary relationships of IDHs, overview. The enzyme belongs to the IDH enzyme family and is clustered into a unique clade among the type II subfamily
Ostreococcus sp. 'lucimarinus'
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Campylobacter curvus
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Campylobacter sp.
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Micromonas commoda
1.1.1.41
metabolism
NAD-IDH is a key enzyme in the tricarboxylic acid cycle. It catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate and CO2, which is accompanied by the reduction of NAD+ to NADH. The IDH reaction provides organisms with not only energy but also biosynthetic precursors, such as 2-oxoglutarate, for metabolism
Ostreococcus sp. 'lucimarinus'
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.8
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
4
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
4
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
4.5
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
10
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
19.3
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
146
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
179
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
220
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
242
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
368
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
444
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.8
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
4
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
4
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
4.5
-
NADP+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'
1.1.1.41
10
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
19.3
-
NAD+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
146
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter curvus
1.1.1.41
179
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Micromonas commoda
1.1.1.41
220
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant D326R/M327H
Ostreococcus sp. 'lucimarinus'
1.1.1.41
242
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Campylobacter sp.
1.1.1.41
368
-
NADP+
pH 75, 25°C, recombinant His6-tagged mutant L584H/D595R
Campylobacter sp.
1.1.1.41
444
-
NAD+
pH 75, 25°C, recombinant His6-tagged enzyme
Ostreococcus sp. 'lucimarinus'