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Literature summary extracted from

  • Yoshida, H.; Sakai, G.; Mori, K.; Kojima, K.; Kamitori, S.; Sode, K.
    Structural analysis of fungus-derived FAD glucose dehydrogenase (2015), Sci. Rep., 5, 13498.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expressed in Escherichia coli strain Origami2 (DE3) Aspergillus flavus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.5.9 in complex with FAD and/or D-glucono-1,5-lactone, sitting-drop vapor diffusion method, using 0.1 M of BisTris, pH 6.5, and 22–25% (w/v) PEG3350 Aspergillus flavus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.9 H505A the mutant enzyme shows drastic decrease in the enzymatic activity Aspergillus flavus
1.1.5.9 H548A the mutant enzyme shows drastic decrease in the enzymatic activity Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Aspergillus flavus B8MX95
-
-
1.1.5.9 Aspergillus flavus ATCC 200026 B8MX95
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 resource Q column chromatography Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Aspergillus flavus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Aspergillus flavus ATCC 200026 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD glucose dehydrogenase
-
 Aspergillus flavus
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Aspergillus flavus
1.1.5.9 FADGDH
-
 Aspergillus flavus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
 Aspergillus flavus