BRENDA - Enzyme Database

Peroxisomal NADP-isocitrate dehydrogenase is required for Arabidopsis stomatal movement

Leterrier, M.; Barroso, J.B.; Valderrama, R.; Begara-Morales, J.C.; Sanchez-Calvo, B.; Chaki, M.; Luque, F.; Vinegla, B.; Palma, J.M.; Corpas, F.J.; Protoplasma 253, 403-415 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
gene pICDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
identification of naturally occurring peroxisomal NADP-ICDH (picdh) Arabidopsis mutant lines, designated as picdh-1 (SALK_072422) and picdh-2 (SALK_039193C) carrying a T-DNA insertion in exons 9 and in the 5'UTR, respectively. The mutation results in a lack of the peroxisomal NADP-ICDH in both picdh-1 and picdh-2 plants. There are no significant differences in phenotype between the wild-type (Wt) and the two mutant (picdh-1 and picdh-2) lines. Intrinsic photosynthetic performance is not altered in the different plants with similar maximum C assimilation capacities, light use efficiency, and respiration rates in darkness due to similar growth rates. No differences are found in growth rates, stomatal densities, or leaf mass-area ratios
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
peroxisome
-
Arabidopsis thaliana
5777
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Arabidopsis thaliana
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
1.1.1.42
isocitrate + NADP+
Arabidopsis thaliana Col-0
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Arabidopsis thaliana
Q9SLK0
-
-
1.1.1.42
Arabidopsis thaliana Col-0
Q9SLK0
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
741388
Arabidopsis thaliana
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
741388
Arabidopsis thaliana Col-0
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Arabidopsis thaliana
1.1.1.42
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
gene pICDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Arabidopsis thaliana
1.1.1.42
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
identification of naturally occurring peroxisomal NADP-ICDH (picdh) Arabidopsis mutant lines, designated as picdh-1 (SALK_072422) and picdh-2 (SALK_039193C) carrying a T-DNA insertion in exons 9 and in the 5'UTR, respectively. The mutation results in a lack of the peroxisomal NADP-ICDH in both picdh-1 and picdh-2 plants. There are no significant differences in phenotype between the wild-type (Wt) and the two mutant (picdh-1 and picdh-2) lines. Intrinsic photosynthetic performance is not altered in the different plants with similar maximum C assimilation capacities, light use efficiency, and respiration rates in darkness due to similar growth rates. No differences are found in growth rates, stomatal densities, or leaf mass-area ratios
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
peroxisome
-
Arabidopsis thaliana
5777
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Arabidopsis thaliana
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
1.1.1.42
isocitrate + NADP+
Arabidopsis thaliana Col-0
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
741388
Arabidopsis thaliana
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
1.1.1.42
isocitrate + NADP+
-
741388
Arabidopsis thaliana Col-0
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
r
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
evolution
Arabidopsis thaliana has three NADP-ICDH genes At1g65930, At5g14590, and At1g54340, coding for the proteins NP_176768, NP_196963, and BT025983, respectively, that are located in different subcellular compartments, i.e. cytosol, chloroplasts, mitochondria, and peroxisomes
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
evolution
Arabidopsis thaliana has three NADP-ICDH genes At1g65930, At5g14590, and At1g54340, coding for the proteins NP_176768, NP_196963, and BT025983, respectively, that are located in different subcellular compartments, i.e. cytosol, chloroplasts, mitochondria, and peroxisomes
Arabidopsis thaliana