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Literature summary extracted from

  • Fujii, T.; Sato, A.; Okamoto, Y.; Yamauchi, T.; Kato, S.; Yoshida, M.; Oikawa, T.; Hata, Y.
    The crystal structure of maleylacetate reductase from Rhizobium sp. strain MTP-10005 provides insights into the reaction mechanism of enzymes in its original family (2016), Proteins, 84, 1029-1042.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.32 sitting drop vapor diffusion method, using 1.1-1.4 M ammonium sulfate, 0.1 M sodium chloride, 2% (w/v) benzamidine-HCl and 0.1 M Na HEPES pH 7.5 at 20°C Rhizobium sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.32 C242A the mutant shows 25% activity compared to the wild type enzyme Rhizobium sp.
1.3.1.32 H243A the mutant shows 0.4% activity compared to the wild type enzyme Rhizobium sp.
1.3.1.32 Y326A the mutant shows 6% activity compared to the wild type enzyme Rhizobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 36406
-
 strain MTP-10005 Rhizobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.32 2-maleylacetate + NADH + H+ Rhizobium sp.
-
 3-oxoadipate + NAD+
-
 ?
1.3.1.32 2-maleylacetate + NADPH + H+ Rhizobium sp.
-
 3-oxoadipate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Rhizobium sp. A1IIX4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.32 0.721
-
 mutant enzyme H243A, at pH 6.5 and 30°C Rhizobium sp.
1.3.1.32 11.7
-
 mutant enzyme Y326A, at pH 6.5 and 30°C Rhizobium sp.
1.3.1.32 50
-
 mutant enzyme C242A, at pH 6.5 and 30°C Rhizobium sp.
1.3.1.32 202
-
 wild type enzyme, at pH 6.5 and 30°C Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NADH + H+
-
 Rhizobium sp. 3-oxoadipate + NAD+
-
 ?
1.3.1.32 2-maleylacetate + NADPH + H+
-
 Rhizobium sp. 3-oxoadipate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 homodimer 2 * 36406, calculated from amino acid sequence Rhizobium sp.

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 GraC
-
 Rhizobium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Rhizobium sp.
1.3.1.32 NADPH
-
 Rhizobium sp.