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Literature summary extracted from

  • Li, H.; Jogl, G.
    Crystal structure of decaprenylphosphoryl-beta-D-ribose 2'-epimerase from Mycobacterium smegmatis (2013), Proteins, 81, 538-543.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.98.3 expressed in Escherichia coli BL21star cells Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.98.3 sitting drop vapor diffusion method, using 15-20% (v/v) isopropanol, 0.2 M sodium citrate pH 6.8, and 0.5% (w/v) N,N-dimethyldodecylamine-N-oxide Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.98.3 44000
-
 x * 44000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.98.3 trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD Mycolicibacterium smegmatis
-
 trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?
1.1.98.3 trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD Mycolicibacterium smegmatis ATCC 700084
-
 trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.3 Mycolicibacterium smegmatis A0R607
-
-
1.1.98.3 Mycolicibacterium smegmatis ATCC 700084 A0R607
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.98.3 nickel-agarose affinity resin column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.98.3 trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD
-
 Mycolicibacterium smegmatis trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?
1.1.98.3 trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD
-
 Mycolicibacterium smegmatis ATCC 700084 trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.98.3 ? x * 44000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
1.1.98.3 decaprenylphosphoryl-beta-D-ribose 2'-epimerase
-
 Mycolicibacterium smegmatis
1.1.98.3 DprE1
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.98.3 FAD
-
 Mycolicibacterium smegmatis