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Literature summary extracted from

  • Zheng, H.; Beliavsky, A.; Tchigvintsev, A.; Brunzelle, J.; Brown, G.; Flick, R.; Evdokimova, E.; Wawrzak, Z.; Mahadevan, R.; Anderson, W.; Savchenko, A.; Yakunin, A.
    Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium (2013), Proteins Struct. Funct. Bioinform., 81, 1031-1041.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.16 structures of apoenzyme and in complex with NAD+, to 1.85 and 1.9 A resolution. Two domain protein with the active site located in the interdomain interface. The NAD+ molecule is bound in the long channel with its nicotinamide ring positioned close to the side chain of the catalytic Cys268 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.16 D426A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 E442A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 F138A decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 K160A Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 K160E Lys160 contributes to the enzyme preference to NAD+, strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 K92A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 N193A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 R145A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 R428A activity similar to wild-type Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 S425A decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 W141A strong decrease in activity Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 Succinic semialdehyde substrate inhibition above 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.022
-
 succinate semialdehyde mutant R428A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.027
-
 succinate semialdehyde wild-type, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.043
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.047
-
 succinate semialdehyde wild-type, cofactor NADP+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.12
-
 succinate semialdehyde mutant F138A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.14
-
 succinate semialdehyde mutant E442A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.15
-
 NAD+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.22
-
 succinate semialdehyde mutant S425A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.23
-
 succinate semialdehyde mutant D426A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.48
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.77
-
 succinate semialdehyde mutant W141A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.4
-
 NADP+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.49
-
 succinate semialdehyde mutant R145A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.6
-
 succinate semialdehyde mutant K92A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 10
-
 NAD+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 11
-
 NAD+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 26
-
 NADP+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 27
-
 NADP+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZPI3
-
-
1.2.1.16 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 Q8ZPI3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + NADH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 succinate + NADH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + NADPH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 succinate + NADPH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 YneI
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.16 0.3
-
 succinate semialdehyde mutant D426A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2 8 succinate semialdehyde mutant R428A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.1
-
 NADP+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.6
-
 NADP+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 14
-
 NAD+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 21
-
 NADP+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 26
-
 succinate semialdehyde mutant F138A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 38
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 39
-
 NAD+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 45
-
 succinate semialdehyde mutant R145A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 46
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 55
-
 succinate semialdehyde wild-type, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 66
-
 succinate semialdehyde mutant W141A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 68
-
 succinate semialdehyde mutant S425A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 73
-
 succinate semialdehyde mutant K92A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 80
-
 NAD+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 83
-
 succinate semialdehyde wild-type, cofactor NADP+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 164
-
 succinate semialdehyde mutant E442A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+ affinity to NAD+ is 10 times higher than to NADP+ Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 NADP+ affinity to NAD+ is 10 times higher than to NADP+ Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.16 0.1
-
 NADP+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.77
-
 NADP+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 1.3
-
 succinate semialdehyde mutant D426A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 3.8
-
 NAD+ mutant K160A , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 7.3
-
 NAD+ mutant K160E , pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 8.6
-
 NADP+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 18
-
 succinate semialdehyde mutant R145A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 28
-
 succinate semialdehyde mutant K92A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 86
-
 succinate semialdehyde mutant W141A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 95
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 97
-
 NAD+ mutant N193A, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 210
-
 succinate semialdehyde mutant F138A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 310
-
 succinate semialdehyde mutant S425A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 880
-
 NAD+ wild-type, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 1100
-
 succinate semialdehyde mutant E442A, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 1300
-
 succinate semialdehyde mutant R428A , cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 1800
-
 succinate semialdehyde wild-type, cofactor NADP+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2000
-
 succinate semialdehyde wild-type, cofactor NAD+, pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium