EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.22 | molecular biology | the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems | Granulibacter bethesdensis |
1.1.1.22 | synthesis | the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems | Granulibacter bethesdensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.22 | recombinant enzyme overexpression | Granulibacter bethesdensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.22 | SDS | strong inhibition | Granulibacter bethesdensis | |
1.1.1.22 | UDP-xylose | allosteric feedback inhibitor, reduces significantly the activity of the enzyme | Granulibacter bethesdensis | |
1.1.1.22 | Urea | strong inhibition | Granulibacter bethesdensis | |
1.1.1.22 | Zn2+ | strong inhibition | Granulibacter bethesdensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.22 | UDP-alpha-D-glucose + 2 NAD+ + H2O | Granulibacter bethesdensis | - |
UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.22 | Granulibacter bethesdensis | - |
originally isolated from the lymph nodes of patients with chronic granulomatous disease (CGD) | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.22 | recombinant enzyme, simple purification method | Granulibacter bethesdensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.22 | UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Granulibacter bethesdensis | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? | |
1.1.1.22 | UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Granulibacter bethesdensis | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.22 | GbUGD | - |
Granulibacter bethesdensis |
1.1.1.22 | UDP-glucose dehydrogenase | - |
Granulibacter bethesdensis |
1.1.1.22 | UDPglucose dehydrogenase | - |
Granulibacter bethesdensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.22 | 37 | - |
recombinant enzyme | Granulibacter bethesdensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.22 | 9 | - |
recombinant enzyme | Granulibacter bethesdensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.22 | additional information | no activity with NADP+ | Granulibacter bethesdensis | |
1.1.1.22 | NAD+ | specific for | Granulibacter bethesdensis |