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Literature summary extracted from

  • Wei, S.; Kulinich, A.; Duan, X.C.; Liu, L.; Voglmeir, J.
    Discovery and biochemical characterization of UDP-glucose dehydrogenase from Granulibacter bethesdensis (2015), Protein Pept. Lett., 22, 628-634.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.22 molecular biology the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems Granulibacter bethesdensis
1.1.1.22 synthesis the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems Granulibacter bethesdensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.22 recombinant enzyme overexpression Granulibacter bethesdensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.22 SDS strong inhibition Granulibacter bethesdensis
1.1.1.22 UDP-xylose allosteric feedback inhibitor, reduces significantly the activity of the enzyme Granulibacter bethesdensis
1.1.1.22 Urea strong inhibition Granulibacter bethesdensis
1.1.1.22 Zn2+ strong inhibition Granulibacter bethesdensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.22 UDP-alpha-D-glucose + 2 NAD+ + H2O Granulibacter bethesdensis
-
UDP-alpha-D-glucuronate + 2 NADH + 2 H+ UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Granulibacter bethesdensis
-
originally isolated from the lymph nodes of patients with chronic granulomatous disease (CGD)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.22 recombinant enzyme, simple purification method Granulibacter bethesdensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 UDP-alpha-D-glucose + 2 NAD+ + H2O
-
Granulibacter bethesdensis UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
?
1.1.1.22 UDP-alpha-D-glucose + 2 NAD+ + H2O
-
Granulibacter bethesdensis UDP-alpha-D-glucuronate + 2 NADH + 2 H+ UDP-glucuronic acid is a key precursor in the biosynthesis of glycoconjugates ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.22 GbUGD
-
Granulibacter bethesdensis
1.1.1.22 UDP-glucose dehydrogenase
-
Granulibacter bethesdensis
1.1.1.22 UDPglucose dehydrogenase
-
Granulibacter bethesdensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.22 37
-
recombinant enzyme Granulibacter bethesdensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.22 9
-
recombinant enzyme Granulibacter bethesdensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.22 additional information no activity with NADP+ Granulibacter bethesdensis
1.1.1.22 NAD+ specific for Granulibacter bethesdensis