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Literature summary extracted from

  • Ibrahim, M.A.; Ghazy, A.H.; Salem, A.M.; Ghazy, M.A.; Abdel-Monsef, M.M.
    Biochemical characterization of buffalo liver glucose-6-phosphate dehydrogenase isoforms (2015), Protein J., 34, 193-204.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.49 Cu2+ potent inhibitor, about 65% residual activity at 2 mM (isoform G6PD2), about 50% residual activity at 2 mM (isoform G6PD1) Bos taurus
1.1.1.49 Fe2+ potent inhibitor, about 10% residual activity at 5 mM (isoform G6PD2), about 30% residual activity at 5 mM (isoform G6PD1) Bos taurus
1.1.1.49 Mn2+ about 10% residual activity at 5 mM (isoform G6PD2) Bos taurus
1.1.1.49 NADPH competitive inhibition Bos taurus
1.1.1.49 Zn2+ potent inhibitor, about 58% residual activity at 5 mM (isoform G6PD2), about 35% residual activity at 2 mM (isoform G6PD1) Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.059
-
 NADP+ isoform G6PD1, at pH 8.0, temperature not specified in the publication Bos taurus
1.1.1.49 0.06
-
 NADP+ isoform G6PD1, at pH 8.0, temperature not specified in the publication Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.49 Co2+ about 110% activity at 5 mM (isoform G6PD1) Bos taurus
1.1.1.49 Mg2+ about 130% activity at 5 mM (isoform G6PD2), about 150% activity at 5 mM (isoform G6PD1) Bos taurus
1.1.1.49 Mn2+ about 112% activity at 2 mM (isoform G6PD1) Bos taurus
1.1.1.49 additional information not influenced by Ca2+ Bos taurus
1.1.1.49 Ni2+ about 110% activity at 5 mM (isoform G6PD2), about 115% activity at 2 mM (isoform G6PD1) Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.49 28000
-
 isoform G6PD1, gel filtration Bos taurus
1.1.1.49 66000
-
 isoform G6PD2, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ Bos taurus
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephacryl S-300 gel filtration, and 2',5'-ADP Sepharose 4B column chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.49 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.49 0.0374
-
 crude extract, at pH 8.0, temperature not specified in the publication Bos taurus
1.1.1.49 0.916
-
 isoform G6PD1, after 24.49fold purification, at pH 8.0, temperature not specified in the publication Bos taurus
1.1.1.49 2.221
-
 isoform G6PD2, after 59.38fold purification, at pH 8.0, temperature not specified in the publication Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Bos taurus 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.49 monomer 1 * 28000, isoform G6PD1, SDS-PAGE Bos taurus
1.1.1.49 monomer 1 * 66000, isoform G6PD2, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G6PD1 isoform Bos taurus
1.1.1.49 G6PD2 isoform Bos taurus
1.1.1.49 glucose-6-phosphate dehydrogenase
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 8
-
 isoform G6PD1 Bos taurus
1.1.1.49 8.2
-
 isoform G6PD2 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.49 0.012
-
 NADPH isoform G6PD1, at pH 8.0, temperature not specified in the publication Bos taurus
1.1.1.49 0.03
-
 NADPH isoform G6PD2, at pH 8.0, temperature not specified in the publication Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.49 Bos taurus isoform G6PD2, isoelectric focusing 5.9 5.7
1.1.1.49 Bos taurus isoform G6PD1, isoelectric focusing 7.9 7.7