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Literature summary extracted from

  • Studts, J.M.; Mitchell, K.H.; Pikus, J.D.; McClay, K.; Steffan, R.J.; Fox, B.G.
    Optimized expression and purification of toluene 4-monooxygenase hydroxylase (2000), Protein Expr. Purif., 20, 58-65.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.236 synthesis by manipulation of vectors and gene inserts to eliminate adventitious catalytic turnover of NADH, up to 60fold increase in the volumetric yield of T4moH activity can be obtained from recombinant fermentations in Escherichia coli BL21(DE3) Pseudomonas mendocina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.236
-
 Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q00456 and Q00457 and Q00460 Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively
-
1.14.13.236 Pseudomonas mendocina KR1 Q00456 and Q00457 and Q00460 Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.236 1250
-
 pH 7.5, 25°C Pseudomonas mendocina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O
-
 ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O
-
 ?