Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Costa, D.M.; Costa, M.A.; Guimaraes, S.L.; Coitinho, J.B.; Gomez, S.V.; Brandao, T.A.; Nagem, R.A.
    A combined approach for enhancing the stability of recombinant cis-dihydrodiol naphthalene dehydrogenase from Pseudomonas putida G7 allowed for the structural and kinetic characterization of the enzyme (2017), Protein Expr. Purif., 132, 50-59.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.29 expressed in Escherichia coli Rosetta (DE3) cells Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.29 hanging drop vapor diffusion method, using 0.2 M calcium chloride dehydrate, 0.1 M HEPES sodium pH 7.5, 28% (w/v) polyethylene glycol 400 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.29 0.006
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 7.0 and 25°C Pseudomonas putida
1.3.1.29 0.012
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 9.4 and 25°C Pseudomonas putida
1.3.1.29 0.31
-
 NAD+ at pH 9.4 and 25°C Pseudomonas putida
1.3.1.29 0.35
-
 NAD+ at pH 7.0 and 25°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.29 27521
-
-
 Pseudomonas putida
1.3.1.29 122000
-
 His6-tagged enzyme, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.29 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ Pseudomonas putida
-
 naphthalene-1,2-diol + NADH + H+
-
 ?
1.3.1.29 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ Pseudomonas putida G7
-
 naphthalene-1,2-diol + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.29 Pseudomonas putida Q9R9Y8
-
-
1.3.1.29 Pseudomonas putida G7 Q9R9Y8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.29 HisTrap column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.29 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Pseudomonas putida naphthalene-1,2-diol + NADH + H+
-
 ?
1.3.1.29 (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 Pseudomonas putida G7 naphthalene-1,2-diol + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.29 homotetramer 4 * 27000, His6-tagged enzyme, SDS-PAGE Pseudomonas putida
1.3.1.29 homotetramer 4 * 29520, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.3.1.29 cis-1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase
-
 Pseudomonas putida
1.3.1.29 cis-dihydrodiol naphthalene dehydrogenase
-
 Pseudomonas putida
1.3.1.29 NahB
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.29 5.6
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 7.0 and 25°C Pseudomonas putida
1.3.1.29 8.3
-
 NAD+ at pH 7.0 and 25°C Pseudomonas putida
1.3.1.29 26.4
-
 (1R,2S)-1,2-dihydronaphthalene-1,2-diol at pH 9.4 and 25°C Pseudomonas putida
1.3.1.29 36.6
-
 NAD+ at pH 9.4 and 25°C Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.29 NAD+
-
 Pseudomonas putida