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Literature summary extracted from

  • Kohler, A.C.; Mills, M.J.L.; Adams, P.D.; Simmons, B.A.; Sale, K.L.
    Structure of aryl O-demethylase offers molecular insight into a catalytic tyrosine-dependent mechanism (2017), Proc. Natl. Acad. Sci. USA, 114, E3205-E3214.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.341 to 1.81 A resolution, structure reveals a unique demethylase fold and a canonical folate-binding domain Sphingomonas paucimobilis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.341 H60A 97-99% loss of activity Sphingomonas paucimobilis
2.1.1.341 M61A 97-99% loss of activity Sphingomonas paucimobilis
2.1.1.341 R122A 97-99% loss of activity Sphingomonas paucimobilis
2.1.1.341 R122A/R147A complete loss of activity Sphingomonas paucimobilis
2.1.1.341 Y247F almost complete loss of activity, no discernible effect on overall protein fold or solution behavior Sphingomonas paucimobilis
2.1.1.341 Y29A 97-99% loss of activity Sphingomonas paucimobilis
2.1.1.341 Y31A 97-99% loss of activity Sphingomonas paucimobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.341 5-methyltetrahydrofolate competitive with respect to tetrahydrofolate, noncompetitive with respect to vanillate Sphingomonas paucimobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.341 0.63
-
vanillate pH 8.0, 30°C Sphingomonas paucimobilis
2.1.1.341 0.72
-
tetrahydrofolate pH 8.0, 30°C Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.341 Sphingomonas paucimobilis Q60FX1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.341 additional information enzyme uses an ordered sequential mechanism Sphingomonas paucimobilis ?
-
?
2.1.1.341 vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.341 LigM
-
Sphingomonas paucimobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.341 30
-
-
Sphingomonas paucimobilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.341 10 40
-
Sphingomonas paucimobilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.341 50
-
inactive above Sphingomonas paucimobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.341 5.76
-
vanillate pH 8.0, 30°C Sphingomonas paucimobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.341 8
-
-
Sphingomonas paucimobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.341 6 8
-
Sphingomonas paucimobilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.341 0.1
-
5-methyltetrahydrofolate substrate vanillate, pH 8.0, 30°C Sphingomonas paucimobilis
2.1.1.341 0.17
-
5-methyltetrahydrofolate substrate tetrahydrofolate, pH 8.0, 30°C Sphingomonas paucimobilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.341 8
-
tetrahydrofolate pH 8.0, 30°C Sphingomonas paucimobilis
2.1.1.341 9.14
-
vanillate pH 8.0, 30°C Sphingomonas paucimobilis