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Literature summary extracted from

  • Agger, J.W.; Isaksen, T.; Várnai, A.; Vidal-Melgosa, S.; Willats, W.G.T.; Ludwig, R.; Horn, S.J.; Eijsink, V.G.H.; Westereng, B.
    Discovery of LPMO activity on hemicelluloses shows the importance of oxidative processes in plant cell wall degradation (2014), Proc. Natl. Acad. Sci. USA, 111, 6287-6292.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.B10 expression in Pichia pastoris Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.B10 Neurospora crassa Q7SHI8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.B10 barely beta-glucan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O dominance of products increasing by three sugars in size, i.e., DP4, DP7, DP10, DP13, and so forth ?
1.14.99.B10 glucomannan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O release of clusters of products up to at least a degree of polymersation DP15, each cluster containing the keto-, gemdiol and native form of hexose oligosaccharides and acetylated species ?
1.14.99.B10 lichenan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 additional information enzyme particularly acts on the glucose backbone of xyloglucan, accepting various substitutions (xylose, galactose) in almost all positions. It requires short stretches of contiguous (1->4)-beta-linked glucose units for activity. No substrates: (1->4)-beta-D-xylan, (1->4)-alpha-D-polygalacturonan, (1->4)-beta-D-mannan, (1->4)-beta-D-galactan, yeast beta-D-glucan, callose, and arabinoxylan Neurospora crassa ?
-
 ?
1.14.99.B10 xyloglucan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O two major oxidized products corresponding to the keto-form of single oxidized species, the most probable product configurations being GoxXXL and GoxXLL ?
1.14.99.B10 xyloglucan 14-mer + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O LPMO9C cuts at the nonreducing end of the intermediate G-unit of a reduced 14-mer derived from tamarind xyloglucan mainly containing XXXGXXXGOH and produces GoxXXXGOH ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.B10 LPMO9C
-
 Neurospora crassa