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Literature summary extracted from

  • Chakraborty, M.; Goel, M.; Chinnadayyala, S.R.; Dahiya, U.R.; Ghosh, S.S.; Goswami, P.
    Molecular characterization and expression of a novel alcohol oxidase from Aspergillus terreus MTCC6324 (2014), PLoS ONE, 9, e95368.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 expressed in Escherichia coli BL21(DE3) cells Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 1.08
-
4-methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 2.88
-
3,4-dimethoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 4.91
-
3-Methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 10.8
-
benzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.7 microsome
-
Aspergillus terreus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.7 74000
-
-
Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Aspergillus terreus I7FDJ2
-
-
1.1.3.7 Aspergillus terreus MTCC6324 I7FDJ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.7 nickel Sepharose 6 column chromatography Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 3,4-dimethoxybenzyl alcohol + O2 i.e. veratryl alcohol Aspergillus terreus 3,4-dimethoxybenzaldehyde + H2O2
-
?
1.1.3.7 3,4-dimethoxybenzyl alcohol + O2 i.e. veratryl alcohol Aspergillus terreus MTCC6324 3,4-dimethoxybenzaldehyde + H2O2
-
?
1.1.3.7 3-methoxybenzyl alcohol + O2 i.e. 3-anisyl alcohol Aspergillus terreus 3-methoxybenzaldehyde + H2O2
-
?
1.1.3.7 3-methoxybenzyl alcohol + O2 i.e. 3-anisyl alcohol Aspergillus terreus MTCC6324 3-methoxybenzaldehyde + H2O2
-
?
1.1.3.7 4-methoxybenzyl alcohol + O2 i.e. 4-anisyl alcohol, best substrate Aspergillus terreus 4-methoxybenzaldehyde + H2O2
-
?
1.1.3.7 4-methoxybenzyl alcohol + O2 i.e. 4-anisyl alcohol, best substrate Aspergillus terreus MTCC6324 4-methoxybenzaldehyde + H2O2
-
?
1.1.3.7 benzyl alcohol + O2 poorest substrate Aspergillus terreus benzaldehyde + H2O2
-
?
1.1.3.7 benzyl alcohol + O2 poorest substrate Aspergillus terreus MTCC6324 benzaldehyde + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 ? x * 76000, SDS-PAGE Aspergillus terreus
1.1.3.7 ? x * 74614, MALDI-TOF mass spectrometry Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 alcohol: O2 oxidoreductase
-
Aspergillus terreus
1.1.3.7 AOX
-
Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.7 30
-
-
Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.7 30 70 the enzyme is stable up to 30°C and the stability is greatly reduced at 40°C (50% inactivation) and leads to 100% inactivation after 5 min at 70°C Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 2.2
-
benzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 105.7
-
3,4-dimethoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 115.4
-
3-Methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 140.9
-
4-methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 6
-
-
Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.7 6 9 the recombinant enzyme demonstrates partial pH stability in range from pH 6.0 to pH 9.0 after 1 h Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD required for activity Aspergillus terreus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.7 Aspergillus terreus isoelectric focusing
-
6.5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 0.2
-
benzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 23.5
-
3-Methoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 39.7
-
3,4-dimethoxybenzyl alcohol recombinant enzyme, at pH 6.0 and 30°C Aspergillus terreus
1.1.3.7 130.5
-
4-methoxybenzyl alcohol at pH 6.0 and 30°C Aspergillus terreus