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Literature summary extracted from

  • Krondorfer, I.; Lipp, K.; Brugger, D.; Staudigl, P.; Sygmund, C.; Haltrich, D.; Peterbauer, C.K.
    Engineering of pyranose dehydrogenase for increased oxygen reactivity (2014), PLoS ONE, 9, e91145.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.29 expressed in Saccharomyces cerevisiae and Pichia pastoris strain X33 Leucoagaricus meleagris

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.29 H103Y the mutant reveals a 5fold increase of the oxygen reactivity compared to the wild type enzyme Leucoagaricus meleagris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 0.02
-
 2,6-dichlorophenolindophenol mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.095
-
 O2 wild type enzyme, with D-glucose as cosubstrate, at pH 7.4 and 30°C Leucoagaricus meleagris
1.1.99.29 0.1
-
 ferrocenium ion mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.14
-
 2,6-dichlorophenolindophenol wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 0.16
-
 ferrocenium ion wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.5
-
 O2 mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 7.4 and 30°C Leucoagaricus meleagris
1.1.99.29 0.69
-
 D-glucose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.07
-
 D-galactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 1.38
-
 1,4-benzoquinone wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 3.64
-
 1,4-benzoquinone mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 3.85
-
 D-glucose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 5.29
-
 D-galactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 128
-
 lactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 395.9
-
 lactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 90000
-
 x * 90000, SDS-PAGE Leucoagaricus meleagris

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Leucoagaricus meleagris Q3L245
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein
-
 Leucoagaricus meleagris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, and gel filtration Leucoagaricus meleagris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 D-galactose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-galactose + ferrocene
-
 ?
1.1.99.29 D-glucose + 1,4-benzoquinone
-
 Leucoagaricus meleagris 2-dehydro-D-glucose + ?
-
 ?
1.1.99.29 D-glucose + 2,6-dichlorophenolindophenol
-
 Leucoagaricus meleagris 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.29 D-glucose + ferrocenium ion
-
 Leucoagaricus meleagris 2-dehydro-D-glucose + ferrocene
-
 ?
1.1.99.29 D-glucose + O2
-
 Leucoagaricus meleagris 2-dehydro-D-glucose + H2O2
-
 ?
1.1.99.29 lactose + ferrocenium ion
-
 Leucoagaricus meleagris ? + ferrocene
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 ? x * 90000, SDS-PAGE Leucoagaricus meleagris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.29 PDH
-
 Leucoagaricus meleagris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.29 2.8
-
 2,6-dichlorophenolindophenol mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 13.6
-
 1,4-benzoquinone mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 24.7
-
 lactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 26
-
 ferrocenium ion mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 27.4
-
 D-glucose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 28.9
-
 D-galactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 37.8
-
 D-glucose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 40.7
-
 2,6-dichlorophenolindophenol wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 41
-
 lactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 47.3
-
 D-galactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 65.4
-
 1,4-benzoquinone wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 130
-
 ferrocenium ion wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD
-
 Leucoagaricus meleagris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.29 0.1
-
 lactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 0.32
-
 lactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 4
-
 1,4-benzoquinone mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 5.5
-
 D-galactose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 7.1
-
 D-glucose mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 44.2
-
 D-galactose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 47.4
-
 1,4-benzoquinone wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 54.8
-
 D-glucose wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 140
-
 2,6-dichlorophenolindophenol mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 260
-
 ferrocenium ion mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris
1.1.99.29 290.7
-
 2,6-dichlorophenolindophenol wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C Leucoagaricus meleagris
1.1.99.29 812.5
-
 ferrocenium ion wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C Leucoagaricus meleagris