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Literature summary extracted from

  • Mahro, M.; Bras, N.F.; Cerqueira, N.M.; Teutloff, C.; Coelho, C.; Romao, M.J.; Leimkuehler, S.
    Identification of crucial amino acids in mouse aldehyde oxidase 3 that determine substrate specificity (2013), PLoS ONE, 8, e82285.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.3.1
-
 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.3.1 F1014I amino acid exchange in the active site Mus musculus
1.2.3.1 F1014L amino acid exchange in the active site, 10fold increase in molybdenim content Mus musculus
1.2.3.1 F1014V amino acid exchange in the active site Mus musculus
1.2.3.1 F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L all residues in the first coordination sphere around the substrate are exchanged to their counterparts in bovine xanthine oxidoreductase. Mutant shows activity towards benzaldehyde, phthalazine and hypoxanthine Mus musculus
1.2.3.1 F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L all residues in the first coordination sphere around the substrate except K889 are exchanged to their counterparts in bovine xanthine oxidoreductase, mutant is devoid of activity towards most substrates tested, while allopurinnol is oxidized at a low rate Mus musculus
1.2.3.1 additional information exchange of several residues in the active site to the ones found in other Aox homologues in mouse or to residues present in bovine xanthine oxidoreductase. Conversion of Aox3 to an xanthine oxidoreductase is achieved exchanging eight residues in the active site. Exchange of the iron-sulfur clusters FeSI, FeSII and both FeSI/FeSII by the corresponding domains of isoform Aox1 results in a decrease in catalytic activity with all substrates tested and both electron acceptor dichlorophenol indophenol and O2 Mus musculus
1.2.3.1 P1015A amino acid exchange in the active site Mus musculus
1.2.3.1 P1015G amino acid exchange in the active site Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.1 0.0014
-
 Phthalazine wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.0022
-
 benzaldehyde mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.0025
-
 benzaldehyde wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.0026
-
 Phthalazine mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.0033
-
 benzaldehyde mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.009
-
 Phthalazine mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.0103
-
 Phthalazine mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.0125
-
 Phthalazine mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.0215
-
 benzaldehyde mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.0227
-
 benzaldehyde mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.032
-
 phenanthridine wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.047
-
 phenanthridine mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.073
-
 phenanthridine mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.087
-
 N1-methyl-nicotineamide mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.089
-
 N1-methyl-nicotineamide mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.099
-
 phenanthridine mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.128
-
 N1-methyl-nicotineamide wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.133
-
 allopurinol mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 0.385
-
 phenanthridine mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.426
-
 allopurinol mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.482
-
 N1-methyl-nicotineamide mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.527
-
 allopurinol mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.733
-
 Phthalazine mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 1.595
-
 allopurinol wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 2.21
-
 benzaldehyde mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 2.93
-
 allopurinol mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 3.86
-
 allopurinol mutant F1014V, pH 8.0,37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.1 Molybdenum
-
 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Mus musculus G3X982 isoform Aox3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 allopurinol + H2O + 2,6-dichlorophenolindophenol
-
 Mus musculus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.3.1 benzaldehyde + H2O + 2,6-dichlorophenolindophenol
-
 Mus musculus benzoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.3.1 N1-methyl-nicotineamide + H2O + 2,6-dichlorophenolindophenol
-
 Mus musculus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.3.1 phenanthridine + H2O + O2
-
 Mus musculus 6-phenanthridone + H2O2
-
 ?
1.2.3.1 phthalazine + H2O + 2,6-dichlorophenol indophenol
-
 Mus musculus ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.3.1 AOX3
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.3.1 0.18
-
 phenanthridine mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.27
-
 allopurinol mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.31
-
 benzaldehyde mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.38
-
 N1-methyl-nicotineamide mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.4
-
 N1-methyl-nicotineamide wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.62
-
 allopurinol mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.77
-
 allopurinol mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.97
-
 Phthalazine mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 1.08
-
 N1-methyl-nicotineamide mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 1.1
-
 Phthalazine wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 1.13
-
 benzaldehyde wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 1.22
-
 allopurinol mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 1.22
-
 allopurinol wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 1.27
-
 benzaldehyde mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 1.42
-
 phenanthridine mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 1.42
-
 phenanthridine wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 1.55
-
 benzaldehyde mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 1.58
-
 allopurinol mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 1.92
-
 N1-methyl-nicotineamide mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 2.13
-
 benzaldehyde mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 2.98
-
 Phthalazine mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 3.9
-
 benzaldehyde mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 4.63
-
 Phthalazine mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 5.78
-
 Phthalazine mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 8.73
-
 Phthalazine mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 8.83
-
 phenanthridine mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 9.68
-
 phenanthridine mutant P1015A, pH 8.0,37°C Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.3.1 [2Fe-2S]-center contains two [2Fe2S] clusters Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.3.1 0.00008
-
 allopurinol mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 0.0001
-
 allopurinol mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.0007
-
 allopurinol wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.0008
-
 N1-methyl-nicotineamide mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.0015
-
 allopurinol mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.0015
-
 allopurinol mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.0018
-
 benzaldehyde mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 0.0027
-
 phenanthridine mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.0031
-
 N1-methyl-nicotineamide wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.0037
-
 phenanthridine mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.0119
-
 allopurinol mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 0.0119
-
 Phthalazine mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C Mus musculus
1.2.3.1 0.0124
-
 N1-methyl-nicotineamide mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.013
-
 benzaldehyde mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.0216
-
 N1-methyl-nicotineamide mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.044
-
 phenanthridine wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.098
-
 benzaldehyde mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.098
-
 phenanthridine mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.11
-
 Phthalazine mutant F1014I, pH 8.0,37°C Mus musculus
1.2.3.1 0.189
-
 phenanthridine mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.37
-
 Phthalazine mutant F1014V, pH 8.0,37°C Mus musculus
1.2.3.1 0.45
-
 benzaldehyde wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 0.47
-
 benzaldehyde mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.56
-
 Phthalazine mutant P1015G, pH 8.0,37°C Mus musculus
1.2.3.1 0.58
-
 benzaldehyde mutant P1015A, pH 8.0,37°C Mus musculus
1.2.3.1 0.79
-
 Phthalazine wild-type, pH 8.0,37°C Mus musculus
1.2.3.1 1.148
-
 Phthalazine mutant P1015A, pH 8.0,37°C Mus musculus