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Literature summary extracted from

  • Cavalier, M.C.; Yim, Y.S.; Asamizu, S.; Neau, D.; Almabruk, K.H.; Mahmud, T.; Lee, Y.H.
    Mechanistic insights into validoxylamine A 7-phosphate synthesis by VldE using the structure of the entire product complex (2012), PLoS ONE, 7, e44934.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.135 expressed in Escherichia coli BL21(DE3) cells Streptomyces hygroscopicus subsp. limoneus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.135 in apo form, in complex with GDP, in complex with GDP and validoxylamine A 7'-phosphate, and in complex with GDP and trehalose, hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 8.0 with 18-35% polyethylene glycol 3350 Streptomyces hygroscopicus subsp. limoneus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.135 GDP-valienol + validamine 7-phosphate Streptomyces hygroscopicus subsp. limoneus
-
 validoxylamine A 7'-phosphate + GDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.135 Streptomyces hygroscopicus subsp. limoneus Q15JG1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.135 Ni-NTA column chromatography, and Superdex 200 gel filtration Streptomyces hygroscopicus subsp. limoneus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.135 GDP-valienol + validamine 7-phosphate
-
 Streptomyces hygroscopicus subsp. limoneus validoxylamine A 7'-phosphate + GDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.135 homodimer x-ray crystallography Streptomyces hygroscopicus subsp. limoneus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.135 validamine-7-phosphate valienyltransferase
-
 Streptomyces hygroscopicus subsp. limoneus
2.5.1.135 vldE
-
 Streptomyces hygroscopicus subsp. limoneus