Literature summary extracted from

Reuter, K.; Pittelkow, M.; Bursy, J.; Heine, A.; Craan, T.; Bremer, E.
Synthesis of 5-hydroxyectoine from ectoine: crystal structure of the non-heme iron(II) and 2-oxoglutarate-dependent dioxygenase EctD (2010), PLoS ONE, 5, e10647.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.11.55	expression in Escherichia coli	Virgibacillus salexigens

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.11.55	crystal structure in complex with Fe3+ at a resolution of 1.85 A. The core of the EctD structure consists of a double-stranded beta-helix forming the main portion of the active-site of the enzyme. The positioning of the iron ligand in the active site is mediated by an evolutionarily conserved 2-His-1-carboxylate iron-binding motif. The side chains of the three residues forming this iron-binding site protrude into a deep cavity in the EctD structure that also harbours the 2-oxoglutarate cosubstrate-binding site	Virgibacillus salexigens

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.11.55	Virgibacillus salexigens	Q2TDY4	-	-

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Release 2023.1 (January 2023)