EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.99.8 | to 2.2 A resolution. The asymmetric unit contains one hetero-trimer, one Mo-PCD (large subunit), one FAD (medium subunit), two [2Fe-2S] clusters (designated as I and II, small subunit), and solvent molecules | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.99.8 | 0.0035 | - |
D-Glyceraldehyde-3-phosphate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.011 | - |
D-glyceraldehyde | cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.012 | - |
D-Glyceraldehyde-3-phosphate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.018 | - |
D-glyceraldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.03 | - |
D-glyceraldehyde | cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.037 | - |
Indoleacetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.085 | - |
D-Glyceraldehyde-3-phosphate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.091 | - |
D-glyceraldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.092 | - |
Indoleacetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.113 | - |
Indoleacetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.138 | - |
D-glyceraldehyde | cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.24 | - |
acetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.63 | - |
D-glyceraldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 1.33 | - |
acetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 1.86 | - |
Indolepyruvate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.99.8 | copper | 0.3 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | copper | 0.56 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | copper | 0.76 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | Iron | 10.5 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | Iron | 18.6 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | Iron | 26.2 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | Molybdenum | 2.9 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | Molybdenum | 4.46 nmol/mg protein | Sulfurisphaera tokodaii | |
1.2.99.8 | Molybdenum | 5.5 nmol/mg protein | Sulfurisphaera tokodaii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.99.8 | 20000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 21000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 29000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 30000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 81000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 82000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 83000 | - |
- |
Sulfurisphaera tokodaii |
1.2.99.8 | 85000 | - |
gel filtration | Sulfurisphaera tokodaii |
1.2.99.8 | 200000 | - |
gel filtration | Sulfurisphaera tokodaii |
1.2.99.8 | 372000 | - |
gel filtration | Sulfurisphaera tokodaii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.99.8 | Sulfurisphaera tokodaii | F9VNL4 and F9VNL6 and F9VNL5 | F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7 | - |
1.2.99.8 | Sulfurisphaera tokodaii | Q96XN5 and Q974U9 and Q974V0 | Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561 | - |
1.2.99.8 | Sulfurisphaera tokodaii | Q96Y29 and Q974U9 and Q974V0 | Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561 | - |
1.2.99.8 | Sulfurisphaera tokodaii DSM 16993 | F9VNL4 and F9VNL6 and F9VNL5 | F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7 | - |
1.2.99.8 | Sulfurisphaera tokodaii DSM 16993 | Q96XN5 and Q974U9 and Q974V0 | Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561 | - |
1.2.99.8 | Sulfurisphaera tokodaii DSM 16993 | Q96Y29 and Q974U9 and Q974V0 | Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561 | - |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.2.99.8 | purified enzyme is dark red in color | Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.99.8 | acetaldehyde + 2,6-dichlorophenolindophenol + H2O | - |
Sulfurisphaera tokodaii | acetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.99.8 | acetaldehyde + 2,6-dichlorophenolindophenol + H2O | - |
Sulfurisphaera tokodaii DSM 16993 | acetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.99.8 | D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol | - |
Sulfurisphaera tokodaii | D-glycerate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.99.8 | D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol | - |
Sulfurisphaera tokodaii DSM 16993 | D-glycerate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.99.8 | D-glyceraldehyde + H2O + methyl viologen | - |
Sulfurisphaera tokodaii | D-glycerate + reduced methyl viologen | - |
? | |
1.2.99.8 | D-glyceraldehyde + H2O + methyl viologen | - |
Sulfurisphaera tokodaii DSM 16993 | D-glycerate + reduced methyl viologen | - |
? | |
1.2.99.8 | D-glyceraldehyde-3-phosphate + H2O + methyl viologen | - |
Sulfurisphaera tokodaii | 3-phospho-D-glycerate + reduced methyl viologen | - |
? | |
1.2.99.8 | D-glyceraldehyde-3-phosphate + H2O + methyl viologen | - |
Sulfurisphaera tokodaii DSM 16993 | 3-phospho-D-glycerate + reduced methyl viologen | - |
? | |
1.2.99.8 | indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O | - |
Sulfurisphaera tokodaii | indoleacetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.99.8 | indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O | - |
Sulfurisphaera tokodaii DSM 16993 | indoleacetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.99.8 | indolepyruvate + 2,6-dichlorophenolindophenol + H2O | - |
Sulfurisphaera tokodaii | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.99.8 | dodecamer | 4 * 83000, gene product ST1781, plus 4 * 29000, gene product ST1783, plus 4 * 20000, gene product ST1782. Isoform Gaor1 is a tetramer of the gene products ST1781/ST1783/ST1782 hetero-trimer | Sulfurisphaera tokodaii |
1.2.99.8 | heterohexamer | 2 * 81000, gene product ST2339, plus 2 * 30000, gene product ST0562, plus 2 * 21000, gene product ST0561. Isoform Gaor1 is a dimer of the gene products ST1781/ST1783/ST1782 heterotrimer | Sulfurisphaera tokodaii |
1.2.99.8 | heterotrimer | 1 * 82000, gene product ST2484, plus 1 * 30000, gene product ST0562, plus 1 * 21000, gene product ST0561 | Sulfurisphaera tokodaii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.99.8 | Gaor1 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | Gaor2 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | Gaor3 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST0561 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST0562 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST1781 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST1782 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST1783 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST2339 | - |
Sulfurisphaera tokodaii |
1.2.99.8 | ST2484 | - |
Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.99.8 | 0.43 | - |
D-Glyceraldehyde-3-phosphate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.65 | - |
D-Glyceraldehyde-3-phosphate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.72 | - |
D-glyceraldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 0.98 | - |
D-Glyceraldehyde-3-phosphate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 2.77 | - |
Indoleacetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 3.23 | - |
acetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 3.38 | - |
acetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 3.46 | - |
Indoleacetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 3.58 | - |
D-glyceraldehyde | cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 3.58 | - |
Indoleacetaldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 3.78 | - |
D-glyceraldehyde | cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 5.23 | - |
D-glyceraldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 11 | - |
Indolepyruvate | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 11.2 | - |
D-glyceraldehyde | cosubstrate methyl viologen, pH 7.0, 70°C | Sulfurisphaera tokodaii | |
1.2.99.8 | 50.5 | - |
D-glyceraldehyde | cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C | Sulfurisphaera tokodaii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.99.8 | FAD | - |
Sulfurisphaera tokodaii | |
1.2.99.8 | molybdopterin cytosine dinucleotide | - |
Sulfurisphaera tokodaii | |
1.2.99.8 | [2Fe-2S]-center | small subunit contains two [2Fe-2S] clusters | Sulfurisphaera tokodaii |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.2.99.8 | Sulfurisphaera tokodaii | isoelectric focusing | - |
6.2 |
1.2.99.8 | Sulfurisphaera tokodaii | isoelectric focusing | - |
6.3 |
1.2.99.8 | Sulfurisphaera tokodaii | isoelectric focusing | - |
6.9 |