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Literature summary extracted from

  • Wakagi, T.; Nishimasu, H.; Miyake, M.; Fushinobu, S.
    Archaeal Mo-containing glyceraldehyde oxidoreductase isozymes exhibit diverse substrate specificities through unique subunit assemblies (2016), PLoS ONE, 11, e0147333.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.99.8 to 2.2 A resolution. The asymmetric unit contains one hetero-trimer, one Mo-PCD (large subunit), one FAD (medium subunit), two [2Fe-2S] clusters (designated as I and II, small subunit), and solvent molecules Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.99.8 0.0035
-
D-Glyceraldehyde-3-phosphate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.011
-
D-glyceraldehyde cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.012
-
D-Glyceraldehyde-3-phosphate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.018
-
D-glyceraldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.03
-
D-glyceraldehyde cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.037
-
Indoleacetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.085
-
D-Glyceraldehyde-3-phosphate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.091
-
D-glyceraldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.092
-
Indoleacetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.113
-
Indoleacetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.138
-
D-glyceraldehyde cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.24
-
acetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.63
-
D-glyceraldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 1.33
-
acetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 1.86
-
Indolepyruvate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.99.8 copper 0.3 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 copper 0.56 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 copper 0.76 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 Iron 10.5 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 Iron 18.6 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 Iron 26.2 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 Molybdenum 2.9 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 Molybdenum 4.46 nmol/mg protein Sulfurisphaera tokodaii
1.2.99.8 Molybdenum 5.5 nmol/mg protein Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.99.8 20000
-
-
Sulfurisphaera tokodaii
1.2.99.8 21000
-
-
Sulfurisphaera tokodaii
1.2.99.8 29000
-
-
Sulfurisphaera tokodaii
1.2.99.8 30000
-
-
Sulfurisphaera tokodaii
1.2.99.8 81000
-
-
Sulfurisphaera tokodaii
1.2.99.8 82000
-
-
Sulfurisphaera tokodaii
1.2.99.8 83000
-
-
Sulfurisphaera tokodaii
1.2.99.8 85000
-
gel filtration Sulfurisphaera tokodaii
1.2.99.8 200000
-
gel filtration Sulfurisphaera tokodaii
1.2.99.8 372000
-
gel filtration Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
1.2.99.8 Sulfurisphaera tokodaii F9VNL4 and F9VNL6 and F9VNL5 F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7
-
1.2.99.8 Sulfurisphaera tokodaii Q96XN5 and Q974U9 and Q974V0 Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
-
1.2.99.8 Sulfurisphaera tokodaii Q96Y29 and Q974U9 and Q974V0 Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
-
1.2.99.8 Sulfurisphaera tokodaii DSM 16993 F9VNL4 and F9VNL6 and F9VNL5 F9VNL4 i.e. gene product ST1781, F9VNL6 i.e. gene product ST1783, F9VNL5 i.e. gene product ST1782, cf. EC 1.2.3.7
-
1.2.99.8 Sulfurisphaera tokodaii DSM 16993 Q96XN5 and Q974U9 and Q974V0 Q96XN5 i.e. gene product ST2484, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
-
1.2.99.8 Sulfurisphaera tokodaii DSM 16993 Q96Y29 and Q974U9 and Q974V0 Q96Y29 i.e. gene product ST2339, Q974U9 i.e. gene product ST0562, Q974V0 i.e. gene product ST0561
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.2.99.8 purified enzyme is dark red in color Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.99.8 acetaldehyde + 2,6-dichlorophenolindophenol + H2O
-
Sulfurisphaera tokodaii acetate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.99.8 acetaldehyde + 2,6-dichlorophenolindophenol + H2O
-
Sulfurisphaera tokodaii DSM 16993 acetate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.99.8 D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol
-
Sulfurisphaera tokodaii D-glycerate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.99.8 D-glyceraldehyde + H2O + 2,6-dichlorophenolindophenol
-
Sulfurisphaera tokodaii DSM 16993 D-glycerate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.99.8 D-glyceraldehyde + H2O + methyl viologen
-
Sulfurisphaera tokodaii D-glycerate + reduced methyl viologen
-
?
1.2.99.8 D-glyceraldehyde + H2O + methyl viologen
-
Sulfurisphaera tokodaii DSM 16993 D-glycerate + reduced methyl viologen
-
?
1.2.99.8 D-glyceraldehyde-3-phosphate + H2O + methyl viologen
-
Sulfurisphaera tokodaii 3-phospho-D-glycerate + reduced methyl viologen
-
?
1.2.99.8 D-glyceraldehyde-3-phosphate + H2O + methyl viologen
-
Sulfurisphaera tokodaii DSM 16993 3-phospho-D-glycerate + reduced methyl viologen
-
?
1.2.99.8 indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O
-
Sulfurisphaera tokodaii indoleacetate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.99.8 indoleacetaldehyde + 2,6-dichlorophenolindophenol + H2O
-
Sulfurisphaera tokodaii DSM 16993 indoleacetate + reduced 2,6-dichlorophenolindophenol
-
?
1.2.99.8 indolepyruvate + 2,6-dichlorophenolindophenol + H2O
-
Sulfurisphaera tokodaii ? + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

EC Number Subunits Comment Organism
1.2.99.8 dodecamer 4 * 83000, gene product ST1781, plus 4 * 29000, gene product ST1783, plus 4 * 20000, gene product ST1782. Isoform Gaor1 is a tetramer of the gene products ST1781/ST1783/ST1782 hetero-trimer Sulfurisphaera tokodaii
1.2.99.8 heterohexamer 2 * 81000, gene product ST2339, plus 2 * 30000, gene product ST0562, plus 2 * 21000, gene product ST0561. Isoform Gaor1 is a dimer of the gene products ST1781/ST1783/ST1782 heterotrimer Sulfurisphaera tokodaii
1.2.99.8 heterotrimer 1 * 82000, gene product ST2484, plus 1 * 30000, gene product ST0562, plus 1 * 21000, gene product ST0561 Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
1.2.99.8 Gaor1
-
Sulfurisphaera tokodaii
1.2.99.8 Gaor2
-
Sulfurisphaera tokodaii
1.2.99.8 Gaor3
-
Sulfurisphaera tokodaii
1.2.99.8 ST0561
-
Sulfurisphaera tokodaii
1.2.99.8 ST0562
-
Sulfurisphaera tokodaii
1.2.99.8 ST1781
-
Sulfurisphaera tokodaii
1.2.99.8 ST1782
-
Sulfurisphaera tokodaii
1.2.99.8 ST1783
-
Sulfurisphaera tokodaii
1.2.99.8 ST2339
-
Sulfurisphaera tokodaii
1.2.99.8 ST2484
-
Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.99.8 0.43
-
D-Glyceraldehyde-3-phosphate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.65
-
D-Glyceraldehyde-3-phosphate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.72
-
D-glyceraldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 0.98
-
D-Glyceraldehyde-3-phosphate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 2.77
-
Indoleacetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 3.23
-
acetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 3.38
-
acetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 3.46
-
Indoleacetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 3.58
-
D-glyceraldehyde cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 3.58
-
Indoleacetaldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 3.78
-
D-glyceraldehyde cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 5.23
-
D-glyceraldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 11
-
Indolepyruvate cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 11.2
-
D-glyceraldehyde cosubstrate methyl viologen, pH 7.0, 70°C Sulfurisphaera tokodaii
1.2.99.8 50.5
-
D-glyceraldehyde cosubstrate 2,6-dichlorophenolindophenol, pH 7.0, 70°C Sulfurisphaera tokodaii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.99.8 FAD
-
Sulfurisphaera tokodaii
1.2.99.8 molybdopterin cytosine dinucleotide
-
Sulfurisphaera tokodaii
1.2.99.8 [2Fe-2S]-center small subunit contains two [2Fe-2S] clusters Sulfurisphaera tokodaii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.99.8 Sulfurisphaera tokodaii isoelectric focusing
-
6.2
1.2.99.8 Sulfurisphaera tokodaii isoelectric focusing
-
6.3
1.2.99.8 Sulfurisphaera tokodaii isoelectric focusing
-
6.9