EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.216 | agriculture | recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control | Persicaria minor |
1.1.1.216 | biotechnology | recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control | Persicaria minor |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.216 | 1,10-phenanthroline | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | 2,2-dipyridyl | 52% inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | 5,5'-dithiobis(2-nitrobenzoic acid) | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Ag+ | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Ca2+ | 24% inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Cu2+ | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | EDTA | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Fe3+ | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | iodoacetamide | 66% inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Li+ | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Mg2+ | 73% inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | additional information | the enzyme activity is inhibited by sulfhydryl reagents, metal-chelating agents and heavy metal ions | Persicaria minor | |
1.1.1.216 | p-chloromercuribenzoate | complete inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Sodium azide | 25% inhibition at 1 mM | Persicaria minor | |
1.1.1.216 | Zn2+ | complete inhibition at 1 mM | Persicaria minor |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.216 | additional information | - |
additional information | Michaelis-Menten kinetics | Persicaria minor | |
1.1.1.216 | 0.17 | - |
(2E,6E)-farnesol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 0.33 | - |
(2Z,6E)-farnesol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 0.42 | - |
(2Z,6Z)-farnesol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 0.71 | - |
carveol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 0.74 | - |
NAD+ | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 0.77 | - |
(S)-perillyl alcohol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 1 | - |
nerodilol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 1.04 | - |
cinnamyl alcohol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 12.5 | - |
geraniol | pH 9.5, 35°C | Persicaria minor | |
1.1.1.216 | 40 | - |
NADP+ | pH 9.5, 35°C | Persicaria minor |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 65000 | - |
- |
Persicaria minor |
1.1.1.216 | 70000 | - |
- |
Persicaria minor |
1.1.1.216 | 130000 | - |
gel filtration | Persicaria minor |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.216 | Persicaria minor | - |
plants growing in an experimental plot at the Institute of Systems Biology of Universiti Kebangsaan Malaysia | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.216 | native enzyme 234fold from leaves to apparent homogeneity by two different steps of anion-exchange chromatography and a cation exchange chromatography step, followed by three successive steps of gel filtration | Persicaria minor |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.216 | leaf | - |
Persicaria minor | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 0.27 | - |
purified native enzyme, pH 9.5, 35°C | Persicaria minor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.216 | (2E,6E)-farnesol + NAD+ | preferred substrates | Persicaria minor | (2E,6E)-farnesal + NADH + H+ | - |
? | |
1.1.1.216 | (2E,6E)-farnesol + NADP+ | NADP+ show 22% of the activity with NAD+ | Persicaria minor | (2E,6E)-farnesal + NADPH + H+ | - |
? | |
1.1.1.216 | (2Z,6E)-farnesol + NAD+ | 66% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | (2Z,6E)-farnesal + NADH + H+ | - |
? | |
1.1.1.216 | (2Z,6Z)-farnesol + NAD+ | 47% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | (2Z,6Z)-farnesal + NADH + H+ | - |
? | |
1.1.1.216 | (S)-perillyl alcohol + NAD+ | 34% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
1.1.1.216 | carveol + NAD+ | 32% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
1.1.1.216 | cinnamyl alcohol + NAD+ | 35% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
1.1.1.216 | geraniol + NAD+ | 37% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
1.1.1.216 | additional information | trans,trans-farnesol is the preferred substrate for the Polygonum minus farnesol dehydrogenase, the geometrical isomers cis,trans-farnesol and cis,cis-farnesol are also oxidized by the enzyme with lower activity. No activity with nerol, p-cumic alcohol, borneol, linalool, menthol, beta-citronellol, 3,7-dihydrolinalool, benzyl alcohol, 2,4-octadien-1-ol, or 2,5-dimethyl-1.5-hexadien-3-ol, tetrahydrogeraniol, tetrahydrolinalool, tetrahydrolavandulol, ethanol, and methanol. Substrate specificity, overview | Persicaria minor | ? | - |
? | |
1.1.1.216 | nerodilol + NAD+ | 36% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.216 | heterodimer | 1 * 65000 + 1 * 70000, SDS-PAGE | Persicaria minor |
1.1.1.216 | More | tryptic peptide mapping and MALDI-TOF/TOF-MS spectrometric analysis, sequence comparisons, overview | Persicaria minor |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.216 | More | cf. EC 1.1.1.354 | Persicaria minor |
1.1.1.216 | NAD(P)+-dependent farnesol dehydrogenase | - |
Persicaria minor |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 35 | - |
- |
Persicaria minor |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 25 | 60 | activity range, profile overview | Persicaria minor |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 30 | 65 | purified native enzyme, 10 min at pH 7.5 in 100 mM tricine-NaOH containing 2.5 mM 2-mercaptoethanol, completely stable up to 30°C, over 90% activity up to 55°C, loss of 80% activity at 60°C, loss of 85% activity at 65°C, and inactivation at 70°C | Persicaria minor |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 9.5 | - |
- |
Persicaria minor |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.216 | 7.5 | 11 | activity range, profile overview. At below pH 8.5 or above pH 10.0, the enzyme activity declines sharply | Persicaria minor |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.216 | additional information | the enzyme utilizes both NAD+ and NADP+ with a strong preference for NAD+ | Persicaria minor | |
1.1.1.216 | NAD+ | - |
Persicaria minor | |
1.1.1.216 | NADP+ | - |
Persicaria minor |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.1.216 | Persicaria minor | isoelectric focusing | - |
6.8 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.216 | physiological function | the enzyme is involved in the biosynthesis of precursors that can be used for the biosynthetic pathway of juvenile hormone III (JH III, methyl-10R,11-epoxy-3,7,11-trimethyl-2E,6E-dodecadienoate). Deployment of transgenic plant with farnesol dehydrogenase enzyme will be beneficial for use in manipulating juvenile hormone biosynthesis in plants | Persicaria minor |