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Literature summary extracted from

  • Ahmad-Sohdi, N.A.; Seman-Kamarulzaman, A.F.; Mohamed-Hussein, Z.A.; Hassan, M.
    Purification and characterization of a novel NAD(P)+-farnesol dehydrogenase from Polygonum minus leaves (2015), PLoS ONE, 10, e0143310.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.216 agriculture recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control Persicaria minor
1.1.1.216 biotechnology recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control Persicaria minor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.216 1,10-phenanthroline complete inhibition at 1 mM Persicaria minor
1.1.1.216 2,2-dipyridyl 52% inhibition at 1 mM Persicaria minor
1.1.1.216 5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 1 mM Persicaria minor
1.1.1.216 Ag+ complete inhibition at 1 mM Persicaria minor
1.1.1.216 Ca2+ 24% inhibition at 1 mM Persicaria minor
1.1.1.216 Cu2+ complete inhibition at 1 mM Persicaria minor
1.1.1.216 EDTA complete inhibition at 1 mM Persicaria minor
1.1.1.216 Fe3+ complete inhibition at 1 mM Persicaria minor
1.1.1.216 iodoacetamide 66% inhibition at 1 mM Persicaria minor
1.1.1.216 Li+ complete inhibition at 1 mM Persicaria minor
1.1.1.216 Mg2+ 73% inhibition at 1 mM Persicaria minor
1.1.1.216 additional information the enzyme activity is inhibited by sulfhydryl reagents, metal-chelating agents and heavy metal ions Persicaria minor
1.1.1.216 p-chloromercuribenzoate complete inhibition at 1 mM Persicaria minor
1.1.1.216 Sodium azide 25% inhibition at 1 mM Persicaria minor
1.1.1.216 Zn2+ complete inhibition at 1 mM Persicaria minor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.216 additional information
-
additional information Michaelis-Menten kinetics Persicaria minor
1.1.1.216 0.17
-
(2E,6E)-farnesol pH 9.5, 35°C Persicaria minor
1.1.1.216 0.33
-
(2Z,6E)-farnesol pH 9.5, 35°C Persicaria minor
1.1.1.216 0.42
-
(2Z,6Z)-farnesol pH 9.5, 35°C Persicaria minor
1.1.1.216 0.71
-
carveol pH 9.5, 35°C Persicaria minor
1.1.1.216 0.74
-
NAD+ pH 9.5, 35°C Persicaria minor
1.1.1.216 0.77
-
(S)-perillyl alcohol pH 9.5, 35°C Persicaria minor
1.1.1.216 1
-
nerodilol pH 9.5, 35°C Persicaria minor
1.1.1.216 1.04
-
cinnamyl alcohol pH 9.5, 35°C Persicaria minor
1.1.1.216 12.5
-
geraniol pH 9.5, 35°C Persicaria minor
1.1.1.216 40
-
NADP+ pH 9.5, 35°C Persicaria minor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.216 65000
-
-
Persicaria minor
1.1.1.216 70000
-
-
Persicaria minor
1.1.1.216 130000
-
gel filtration Persicaria minor

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.216 Persicaria minor
-
plants growing in an experimental plot at the Institute of Systems Biology of Universiti Kebangsaan Malaysia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.216 native enzyme 234fold from leaves to apparent homogeneity by two different steps of anion-exchange chromatography and a cation exchange chromatography step, followed by three successive steps of gel filtration Persicaria minor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.216 leaf
-
Persicaria minor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.216 0.27
-
purified native enzyme, pH 9.5, 35°C Persicaria minor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.216 (2E,6E)-farnesol + NAD+ preferred substrates Persicaria minor (2E,6E)-farnesal + NADH + H+
-
?
1.1.1.216 (2E,6E)-farnesol + NADP+ NADP+ show 22% of the activity with NAD+ Persicaria minor (2E,6E)-farnesal + NADPH + H+
-
?
1.1.1.216 (2Z,6E)-farnesol + NAD+ 66% of the activity with 2-trans,6-trans-farnesol Persicaria minor (2Z,6E)-farnesal + NADH + H+
-
?
1.1.1.216 (2Z,6Z)-farnesol + NAD+ 47% of the activity with 2-trans,6-trans-farnesol Persicaria minor (2Z,6Z)-farnesal + NADH + H+
-
?
1.1.1.216 (S)-perillyl alcohol + NAD+ 34% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
?
1.1.1.216 carveol + NAD+ 32% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
?
1.1.1.216 cinnamyl alcohol + NAD+ 35% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
?
1.1.1.216 geraniol + NAD+ 37% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
?
1.1.1.216 additional information trans,trans-farnesol is the preferred substrate for the Polygonum minus farnesol dehydrogenase, the geometrical isomers cis,trans-farnesol and cis,cis-farnesol are also oxidized by the enzyme with lower activity. No activity with nerol, p-cumic alcohol, borneol, linalool, menthol, beta-citronellol, 3,7-dihydrolinalool, benzyl alcohol, 2,4-octadien-1-ol, or 2,5-dimethyl-1.5-hexadien-3-ol, tetrahydrogeraniol, tetrahydrolinalool, tetrahydrolavandulol, ethanol, and methanol. Substrate specificity, overview Persicaria minor ?
-
?
1.1.1.216 nerodilol + NAD+ 36% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.216 heterodimer 1 * 65000 + 1 * 70000, SDS-PAGE Persicaria minor
1.1.1.216 More tryptic peptide mapping and MALDI-TOF/TOF-MS spectrometric analysis, sequence comparisons, overview Persicaria minor

Synonyms

EC Number Synonyms Comment Organism
1.1.1.216 More cf. EC 1.1.1.354 Persicaria minor
1.1.1.216 NAD(P)+-dependent farnesol dehydrogenase
-
Persicaria minor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.216 35
-
-
Persicaria minor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.216 25 60 activity range, profile overview Persicaria minor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.216 30 65 purified native enzyme, 10 min at pH 7.5 in 100 mM tricine-NaOH containing 2.5 mM 2-mercaptoethanol, completely stable up to 30°C, over 90% activity up to 55°C, loss of 80% activity at 60°C, loss of 85% activity at 65°C, and inactivation at 70°C Persicaria minor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.216 9.5
-
-
Persicaria minor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.216 7.5 11 activity range, profile overview. At below pH 8.5 or above pH 10.0, the enzyme activity declines sharply Persicaria minor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.216 additional information the enzyme utilizes both NAD+ and NADP+ with a strong preference for NAD+ Persicaria minor
1.1.1.216 NAD+
-
Persicaria minor
1.1.1.216 NADP+
-
Persicaria minor

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.216 Persicaria minor isoelectric focusing
-
6.8

General Information

EC Number General Information Comment Organism
1.1.1.216 physiological function the enzyme is involved in the biosynthesis of precursors that can be used for the biosynthetic pathway of juvenile hormone III (JH III, methyl-10R,11-epoxy-3,7,11-trimethyl-2E,6E-dodecadienoate). Deployment of transgenic plant with farnesol dehydrogenase enzyme will be beneficial for use in manipulating juvenile hormone biosynthesis in plants Persicaria minor