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Literature summary extracted from

  • Foumani, M.; Vuong, T.V.; MacCormick, B.; Master, E.R.
    Enhanced polysaccharide binding and activity on linear beta-glucans through addition of carbohydrate-binding modules to either terminus of a glucooligosaccharide oxidase (2015), PLoS ONE, 10, e0125398.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.B3 expressed as fusion protein with carbohydrate binding modules CBM3, CBM11, and CBM44 from Clostridium thermocellum in Pichia pastoris GS115 cells Sarocladium strictum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.B3 0.07
-
 cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.B3 Sarocladium strictum D7PF15
-
-
1.1.99.B3 Sarocladium strictum CBS 346.70 D7PF15
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.B3 Ni-NTA resin column chromatography Sarocladium strictum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.B3 Avicel + O2
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 Avicel + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
1.1.99.B3 beta-(1->3,1->4)-glucan + O2
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 beta-(1->3,1->4)-glucan + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
1.1.99.B3 carboxymethyl cellulose + O2
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 carboxymethyl cellulose + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
1.1.99.B3 cellotetraose + O2
-
 Sarocladium strictum cellotetraono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellotetraose + O2
-
 Sarocladium strictum CBS 346.70 cellotetraono-1,5-lactone + H2O2
-
 ?
1.1.99.B3 cellulose + O2
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 cellulose + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
1.1.99.B3 glucomannan + O2
-
 Sarocladium strictum ?
-
 ?
1.1.99.B3 xyloglucan + O2
-
 Sarocladium strictum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.B3 GOOX
-
 Sarocladium strictum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.B3 4.17
-
 cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.B3 56.7
-
 cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum