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Literature summary extracted from

  • Leland, T.J.; Hanson, A.D.
    Induction of a specific N-methyltransferase enzyme by long-term heat stress during barley leaf growth (1985), Plant Physiol., 79, 451-457.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.340 potassium phosphate
-
 Hordeum vulgare
2.1.1.340 sodium carbonate
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.340 43000
-
 1 * 43000, SDS-PAGE Hordeum vulgare
2.1.1.340 45000
-
 gel filtration Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.340 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.340
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.340 leaf first leaves synthesize gramine constitutively Hordeum vulgare
-
2.1.1.340 additional information no activity in seedling Hordeum vulgare
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.340 freeze-thawing reduces activity by 50% Hordeum vulgare
2.1.1.340 leaf extract, 4°C, 75% residual activity after 4 days Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.340 2 S-adenosyl-L-methionine + 3-aminomethylindole
-
 Hordeum vulgare 2 S-adenosyl-L-homocysteine + gramine overall reaction ?
2.1.1.340 2 S-adenosyl-L-methionine + N-methyl-3-aminomethylindole
-
 Hordeum vulgare 2 S-adenosyl-L-homocysteine + (1H-indol-3-yl)-N,N-dimethylmethanamine
-
 ?
2.1.1.340 additional information no substrate: tryptamine. Poor substrate: tyramine Hordeum vulgare ?
-
 ?
2.1.1.340 S-adenosyl-L-methionine + 3-aminomethylindole
-
 Hordeum vulgare S-adenosyl-L-homocysteine + N-methyl-3-aminomethylindole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.340 monomer 1 * 43000, SDS-PAGE Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.340 9
-
-
 Hordeum vulgare

General Information

EC Number General Information Comment Organism
2.1.1.340 physiological function activity in expanding fifth leaves is increased 8- to 20fold by growth at high temperatures(35°C day/30°C night) compared to cool temperatures (15°C/10°C). Several days of high temperature are required for full induction of activity. No induction occurs in leaves which completed expansion in cool conditions before exposure to high temperature Hordeum vulgare