EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.17 | dithiothreitol | isoform MAM3 has no requirement for dithiothreitol | Arabidopsis thaliana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.17 | expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.17 | ATP | - |
Arabidopsis thaliana | |
2.3.3.17 | EDTA | complete inhibition at 0.04 mM | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.17 | 0.081 | - |
9-methylthio-2-oxononanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.253 | - |
8-methylthio-2-oxooctanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.463 | - |
6-methylthio-2-oxohexanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.476 | - |
5-methylthio-2-oxopentanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.932 | - |
4-methylthio-2-oxobutanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 1 | - |
2-oxoisovalerate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 2.3 | - |
acetyl-CoA | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 8.6 | - |
pyruvate | at pH 8.0 and 32°C | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.3.17 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.17 | Mn2+ | highest activity with Mn2+ | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.17 | 53000 | - |
x * 53000, SDS-PAGE | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.17 | Arabidopsis thaliana | Q9FN52 | ecotype Columbia | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.17 | Ni-NTA column chromatography | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.17 | 2-oxoisovalerate + acetyl-CoA | - |
Arabidopsis thaliana | isopropylmalate + CoA | - |
? | |
2.3.3.17 | 4-methyl-2-oxopentanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
2.3.3.17 | 4-methylthio-2-oxobutanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 2-(2'-methylthioethyl)-malate + CoA | - |
? | |
2.3.3.17 | 5-methyl-2-oxohexanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
2.3.3.17 | 5-methylthio-2-oxopentanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
2.3.3.17 | 6-methylthio-2-oxohexanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 2-(4-methylthiobutyl)malate + CoA | - |
? | |
2.3.3.17 | 8-methylthio-2-oxooctanoate + acetyl-CoA + H2O | - |
Arabidopsis thaliana | 2-(6-methylthiohexyl)malate + CoA | - |
? | |
2.3.3.17 | 9-methylthio-2-oxononanoate + acetyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
2.3.3.17 | additional information | 2-oxo-dodecanoate is not a substrate for isoform MAM3 | Arabidopsis thaliana | ? | - |
? | |
2.3.3.17 | pyruvate + acetyl-CoA | - |
Arabidopsis thaliana | citramalate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.3.17 | ? | x * 53000, SDS-PAGE | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.17 | MAM3 | isoform, formerly known as MAM-L | Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.17 | 32 | - |
- |
Arabidopsis thaliana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.17 | 25 | 39 | 70% activity at 25°C and 39°C | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.17 | 0.03 | - |
9-methylthio-2-oxononanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.17 | - |
pyruvate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.18 | - |
2-oxoisovalerate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.3 | - |
8-methylthio-2-oxooctanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 1.3 | - |
4-methylthio-2-oxobutanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 1.3 | - |
5-methylthio-2-oxopentanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 2.5 | - |
6-methylthio-2-oxohexanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 3 | - |
acetyl-CoA | at pH 8.0 and 32°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.17 | 8 | - |
- |
Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.17 | 6.3 | 9.2 | 50% activity at pH 6.3 and 9.0 | Arabidopsis thaliana |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.3.3.17 | 0.01 | - |
at pH 8.0 and 32°C | Arabidopsis thaliana | EDTA | |
2.3.3.17 | 18 | - |
at pH 8.0 and 32°C | Arabidopsis thaliana | ATP |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.3.17 | metabolism | isoform MAM3 catalyzes the formation of all glucosinolate chain lengths in vivo | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.17 | 0.023 | - |
pyruvate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.2 | - |
2-oxoisovalerate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 0.37 | - |
9-methylthio-2-oxononanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 1.28 | - |
8-methylthio-2-oxooctanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 1.3 | - |
acetyl-CoA | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 1.38 | - |
4-methylthio-2-oxobutanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 2.73 | - |
5-methylthio-2-oxopentanoate | at pH 8.0 and 32°C | Arabidopsis thaliana | |
2.3.3.17 | 5.4 | - |
6-methylthio-2-oxohexanoate | at pH 8.0 and 32°C | Arabidopsis thaliana |