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Literature summary extracted from

  • Textor, S.; de Kraker, J.W.; Hause, B.; Gershenzon, J.; Tokuhisa, J.G.
    MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis (2007), Plant Physiol., 144, 60-71.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.17 dithiothreitol isoform MAM3 has no requirement for dithiothreitol Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.17 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.17 ATP
-
 Arabidopsis thaliana
2.3.3.17 EDTA complete inhibition at 0.04 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.17 0.081
-
 9-methylthio-2-oxononanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.253
-
 8-methylthio-2-oxooctanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.463
-
 6-methylthio-2-oxohexanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.476
-
 5-methylthio-2-oxopentanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.932
-
 4-methylthio-2-oxobutanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 1
-
 2-oxoisovalerate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 2.3
-
 acetyl-CoA at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 8.6
-
 pyruvate at pH 8.0 and 32°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.17 chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.17 Mn2+ highest activity with Mn2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.17 53000
-
 x * 53000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.17 Arabidopsis thaliana Q9FN52 ecotype Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.17 Ni-NTA column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.17 2-oxoisovalerate + acetyl-CoA
-
 Arabidopsis thaliana isopropylmalate + CoA
-
 ?
2.3.3.17 4-methyl-2-oxopentanoate + acetyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.3.17 4-methylthio-2-oxobutanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(2'-methylthioethyl)-malate + CoA
-
 ?
2.3.3.17 5-methyl-2-oxohexanoate + acetyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.3.17 5-methylthio-2-oxopentanoate + acetyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.3.17 6-methylthio-2-oxohexanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(4-methylthiobutyl)malate + CoA
-
 ?
2.3.3.17 8-methylthio-2-oxooctanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(6-methylthiohexyl)malate + CoA
-
 ?
2.3.3.17 9-methylthio-2-oxononanoate + acetyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.3.17 additional information 2-oxo-dodecanoate is not a substrate for isoform MAM3 Arabidopsis thaliana ?
-
 ?
2.3.3.17 pyruvate + acetyl-CoA
-
 Arabidopsis thaliana citramalate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.17 ? x * 53000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.3.17 MAM3 isoform, formerly known as MAM-L Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.17 32
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.17 25 39 70% activity at 25°C and 39°C Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.17 0.03
-
 9-methylthio-2-oxononanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.17
-
 pyruvate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.18
-
 2-oxoisovalerate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.3
-
 8-methylthio-2-oxooctanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 1.3
-
 4-methylthio-2-oxobutanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 1.3
-
 5-methylthio-2-oxopentanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 2.5
-
 6-methylthio-2-oxohexanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 3
-
 acetyl-CoA at pH 8.0 and 32°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.17 8
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.17 6.3 9.2 50% activity at pH 6.3 and 9.0 Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.3.17 0.01
-
 at pH 8.0 and 32°C Arabidopsis thaliana EDTA
2.3.3.17 18
-
 at pH 8.0 and 32°C Arabidopsis thaliana ATP

General Information

EC Number General Information Comment Organism
2.3.3.17 metabolism isoform MAM3 catalyzes the formation of all glucosinolate chain lengths in vivo Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.3.17 0.023
-
 pyruvate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.2
-
 2-oxoisovalerate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 0.37
-
 9-methylthio-2-oxononanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 1.28
-
 8-methylthio-2-oxooctanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 1.3
-
 acetyl-CoA at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 1.38
-
 4-methylthio-2-oxobutanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 2.73
-
 5-methylthio-2-oxopentanoate at pH 8.0 and 32°C Arabidopsis thaliana
2.3.3.17 5.4
-
 6-methylthio-2-oxohexanoate at pH 8.0 and 32°C Arabidopsis thaliana