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Literature summary extracted from

  • Bak, S.; Feyereisen, R.
    The involvement of two p450 enzymes, CYP83B1 and CYP83A1, in auxin homeostasis and glucosinolate biosynthesis (2001), Plant Physiol., 127, 108-118.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.43 expression in Saccharomyces cerevisiae Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.45 tryptamine Ks value 18 microM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.43 0.15
-
 indole-3-acetaldoxime pH not specified in the publication, temperature not specified in the publication Arabidopsis thaliana
1.14.14.45 0.0031
-
 (E)-indol-3-ylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.45 (E)-indol-3-ylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2 Arabidopsis thaliana
-
 S-[(E)-N-hydroxy(indol-3-yl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
1.14.14.45 (E)-p-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2 Arabidopsis thaliana
-
 S-[(Z)-N-hydroxy(p-hydroxyphenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
1.14.14.45 (E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2 Arabidopsis thaliana
-
 S-[(Z)-N-hydroxy(phenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.43 Arabidopsis thaliana P48421
-
-
1.14.14.45 Arabidopsis thaliana O65782
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.43 (E)-4-hydroxyphenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 1-aci-nitro-2-(4-hydroxyphenyl)-ethane + H2O + [oxidized NADPH-hemoprotein reductase] first step of reaction ?
1.14.14.43 (E)-phenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 1-aci-nitro-2-phenylethane + H2O + [oxidized NADPH-hemoprotein reductase] first step of reaction ?
1.14.14.43 1-aci-nitro-2-(4-hydroxyphenyl)-ethane + 2-mercaptoethanol
-
 Arabidopsis thaliana (Z)-2-hydroxyethyl N-hydroxy-2-(4-hydroxyphenyl)ethanimidothioate + H2O
-
 ?
1.14.14.43 indole-3-acetaldoxime + O2 + glutathione + [reduced NADPH-hemoprotein reductase] indole-3-acetaldoxime is the physiological substrate for isoform CYP83B1 but not for CYP83A1 Arabidopsis thaliana 1-aci-nitro-2-indolyl-ethane + H2O + [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.43 additional information isoform CYP83A1 catalyzes the initial conversion of aldoximes to thiohydroximates in the synthesis of glucosinolates not derived from tryptophan Arabidopsis thaliana ?
-
 ?
1.14.14.45 (E)-indol-3-ylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
 Arabidopsis thaliana S-[(E)-N-hydroxy(indol-3-yl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
1.14.14.45 (E)-p-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
 Arabidopsis thaliana S-[(Z)-N-hydroxy(p-hydroxyphenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
1.14.14.45 (E)-phenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + glutathione + O2
-
 Arabidopsis thaliana S-[(Z)-N-hydroxy(phenyl)acetimidoyl]-L-glutathione + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.43 CYP83A1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.45 0.16
-
 (E)-p-hydroxyphenylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana
1.14.14.45 0.25
-
 (E)-phenylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana
1.14.14.45 0.43
-
 (E)-indol-3-ylacetaldehyde oxime pH 8.1, 29°C Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.14.43 physiological function ectopic overexpression of CYP83A1 rescues the rnt1-1 phenotype caused by knockout of CYP83B1 and showing a strong auxin excess phenotype Arabidopsis thaliana
1.14.14.45 physiological function lack of the CYP83B1 gene product leads to auxin excess and indole glucosinolate deficit, ectopic overexpression of CYP83A1 using a 35S promoter rescues phenotype. Indole-3-acetaldoxime has a 50-fold higher affinity toward CYP83B1 than toward CYP83A1, EC 1.14.14.43. Both enzymes also metabolize the phenylalanine- and tyrosine-derived aldoximes. Indole-3-acetaldoxime is the physiological substrate for CYP83B1 Arabidopsis thaliana