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Literature summary extracted from

  • Gu, W.; Geng, C.; Xue, W.; Wu, Q.; Chao, J.; Xu, F.; Sun, H.; Jiang, L.; Han, Y.; Zhang, S.
    Characterization and function of the 3-hydroxy-3-methylglutaryl-CoA reductase gene in Alisma orientale (Sam.) Juz. and its relationship with protostane triterpene production (2015), Plant Physiol. Biochem., 97, 378-389.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 gene HMGR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain BL21(DE3) Rosetta, quantitative realtime-PCR enzyme expression analysis Alisma plantago-aquatica subsp. orientale

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 Alisol B 23-actetate
-
 Alisma plantago-aquatica subsp. orientale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.34 64600
-
-
 Alisma plantago-aquatica subsp. orientale
1.1.1.34 66500
-
-
 Alisma plantago-aquatica subsp. orientale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 (R)-mevalonate + CoA + 2 NADP+ Alisma plantago-aquatica subsp. orientale
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 r
1.1.1.34 additional information Alisma plantago-aquatica subsp. orientale the enzyme is involved in the synthesis of the protostane triterpene Alisol B 23-acetate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Alisma plantago-aquatica subsp. orientale F1DTB9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.34 recombinant solubilized enzyme from Escherichia coli strain BL21(DE3) Rosetta by nickel affinity chromatography and dialysis Alisma plantago-aquatica subsp. orientale

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.34 recombinant enzyme from inclusion bodies after expression in Escherichia coli strain BL21(DE3) Rosetta Alisma plantago-aquatica subsp. orientale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 (R)-mevalonate + CoA + 2 NADP+
-
 Alisma plantago-aquatica subsp. orientale (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 r
1.1.1.34 additional information the enzyme is involved in the synthesis of the protostane triterpene Alisol B 23-acetate Alisma plantago-aquatica subsp. orientale ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.34 ? x * 64600, about, sequence calculation, x * 66500, recombinant enzyme, SDS-PAGE Alisma plantago-aquatica subsp. orientale
1.1.1.34 More tertiary structure modelling, overview Alisma plantago-aquatica subsp. orientale

Synonyms

EC Number Synonyms Comment Organism
1.1.1.34 3-hydroxy-3-methylglutaryl-CoA reductase
-
 Alisma plantago-aquatica subsp. orientale
1.1.1.34 HMGR
-
 Alisma plantago-aquatica subsp. orientale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.34 30
-
 assay at, reverse reaction Alisma plantago-aquatica subsp. orientale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 7.2
-
 assay at, reverse reaction Alisma plantago-aquatica subsp. orientale

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADP+
-
 Alisma plantago-aquatica subsp. orientale
1.1.1.34 NADPH
-
 Alisma plantago-aquatica subsp. orientale

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.34 Alisma plantago-aquatica subsp. orientale sequence calculation
-
 7.87

General Information

EC Number General Information Comment Organism
1.1.1.34 metabolism the enzyme a key enzyme in the synthetic pathway of protostane triterpenes, including Alisol B 23-acetate and its derivatives Alisma plantago-aquatica subsp. orientale