Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cardi, M.; Chibani, K.; Castiglia, D.; Cafasso, D.; Pizzo, E.; Rouhier, N.; Jacquot, J.P.; Esposito, S.
    Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots (2013), Plant Physiol. Biochem., 73, 266-273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.49 expressed in Escherichia coli BL21(DE3) cells Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.49 NADPH
-
 Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.0066
-
 NADP+ native enzyme, pH and temperature not specified in the publication Hordeum vulgare
1.1.1.49 0.015
-
 NADP+ recombinant His-tagged enzyme, pH and temperature not specified in the publication Hordeum vulgare
1.1.1.49 0.78
-
 D-glucose 6-phosphate native enzyme, pH and temperature not specified in the publication Hordeum vulgare
1.1.1.49 0.86
-
 D-glucose 6-phosphate recombinant His-tagged enzyme, pH and temperature not specified in the publication Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.49 plastid
-
 Hordeum vulgare 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.49 55800
-
-
 Hordeum vulgare
1.1.1.49 57000
-
-
 Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ Hordeum vulgare
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.49 dithiothreitol the recombinant His-tagged enzyme shows 60% residual activity after 1 h at 25 mM dithiothreitol Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Hordeum vulgare
-
 cultivar Nure
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 Blue-Sepharose column chromatography, Resource-Q column chromatography, and HiTrap column chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.49 root
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Hordeum vulgare 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.49 ? x * 55800, calculated from amino acid sequence Hordeum vulgare
1.1.1.49 ? x * 57000, native enzyme, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 P2-G6PDH
-
 Hordeum vulgare
1.1.1.49 plastidial glucose 6-phosphate dehydrogenase
-
 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.49 0.066
-
 NADPH recombinant His-tagged enzyme, pH and temperature not specified in the publication Hordeum vulgare
1.1.1.49 0.095
-
 NADPH native enzyme, pH and temperature not specified in the publication Hordeum vulgare