EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.360 | expressed in Escherichia coli Rosetta 2(DE3) | Citrus unshiu |
2.4.1.360 | expressed in Escherichia coli Rosetta 2(DE3) | Citrus japonica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.360 | 0.00085 | - |
2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 0.0144 | - |
nothofagin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 0.0715 | - |
UDP-alpha-D-glucose | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor | Citrus japonica | |
2.4.1.360 | 0.112 | - |
6-beta-D-glucopyranosyl-2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.360 | Citrus japonica | - |
- |
- |
2.4.1.360 | Citrus unshiu | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.360 | - |
Citrus unshiu |
2.4.1.360 | purification via a nickel-affinity column, concentration using Amicon-Ultra-15 Ultracel-10k | Citrus japonica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.360 | flower | - |
Citrus japonica | - |
2.4.1.360 | leaf | - |
Citrus unshiu | - |
2.4.1.360 | leaf | - |
Citrus japonica | - |
2.4.1.360 | additional information | detected in all plant organs | Citrus unshiu | - |
2.4.1.360 | additional information | detected in all plant organs | Citrus japonica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.360 | 2 UDP-alpha-D-glucose + 2-hydroxynaringenin | - |
Citrus unshiu | 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin | - |
? | |
2.4.1.360 | 2 UDP-alpha-D-glucose + 2-hydroxynaringenin | - |
Citrus japonica | 2 UDP + 6,8-di-(beta-D-glucopyranosyl)-2-hydroxynaringenin | - |
? | |
2.4.1.360 | 2 UDP-alpha-D-glucose + phloretin | - |
Citrus unshiu | 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin | - |
? | |
2.4.1.360 | 2 UDP-alpha-D-glucose + phloretin | - |
Citrus japonica | 2 UDP + 3',5'-di-(beta-D-glucopyranosyl)phloretin | - |
? | |
2.4.1.360 | additional information | no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin | Citrus unshiu | ? | - |
? | |
2.4.1.360 | additional information | no activity with naringenin, apigenin, quercetin, isoliquiritigenin, propyl gallate, and 2',4'dihydroxyacetophenone suggesting that 2',4',6'-trihydroxyacetophenone-like structures are necessary for substrates to be acceptable. Very low activity with phloridzin | Citrus japonica | ? | - |
? | |
2.4.1.360 | UDP-alpha-D-galactose + 2-hydroxypinocembrin | low activity with sugar donor UDP-alpha-D-galactose | Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-galactose + 2-hydroxypinocembrin | low activity with sugar donor UDP-alpha-D-galactose | Citrus japonica | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone | - |
Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + 2',4',6'-trihydroxyacetophenone | - |
Citrus japonica | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde | - |
Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + 2,4,6-trihydroxybenzaldehyde | - |
Citrus japonica | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone | - |
Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + 2-phenyl-2',4',6'-trihydroxyacetophenone | - |
Citrus japonica | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + maclurin | - |
Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + maclurin | - |
Citrus japonica | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + nothofagin | - |
Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-glucose + nothofagin | - |
Citrus japonica | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin | - |
Citrus unshiu | UDP + ? | - |
? | |
2.4.1.360 | UDP-alpha-D-xylose + 6-beta-D-glucopyranosyl-2-hydroxynaringenin | - |
Citrus japonica | UDP + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.360 | CuCGT | - |
Citrus unshiu |
2.4.1.360 | FcCGT | - |
Citrus japonica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.360 | 45 | - |
- |
Citrus unshiu |
2.4.1.360 | 50 | - |
- |
Citrus japonica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.360 | 55 | - |
inactivation above | Citrus unshiu |
2.4.1.360 | 60 | - |
activity is significantly retained up to 60°C | Citrus japonica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.360 | 0.8 | - |
UDP-alpha-D-glucose | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor | Citrus japonica | |
2.4.1.360 | 5.3 | - |
nothofagin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 5.7 | - |
6-beta-D-glucopyranosyl-2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 7.6 | - |
2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 12 | - |
phloretin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.360 | 8 | 11 | more than 65% of maximum activity in this range | Citrus unshiu |
2.4.1.360 | 8 | 11 | more than 65% of maximum activity in this range | Citrus japonica |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.360 | 0.0089 | - |
2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 0.037 | - |
nothofagin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica | |
2.4.1.360 | 0.11 | - |
UDP-alpha-D-glucose | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C, nothofagin as sugar acceptor | Citrus japonica | |
2.4.1.360 | 0.51 | - |
6-beta-D-glucopyranosyl-2-hydroxynaringenin | 50 mM potassium phosphate buffer, pH 8.0, containing 5 mM 2-mercaptoethanol and 0.01% BSA, 30°C | Citrus japonica |