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Literature summary extracted from

  • Bouvier D'Yvoire, M.; Bouchabke-Coussa, O.; Voorend, W.; Antelme, S.; Cezard, L.; Legee, F.; Lebris, P.; Legay, S.; Whitehead, C.; McQueen-Mason, S.; Gomez, L.; Jouanin, L.; Lapierre, C.; Sibout, R.
    Disrupting the cinnamyl alcohol dehydrogenase 1 gene (BdCAD1) leads to altered lignification and improved saccharification in Brachypodium distachyon (2013), Plant J., 73, 496-508.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 gene BdCAD1, DNA and amino acid sequence determination of wild-type and mutant genes, genotyping, phylogenetic analysis and tree, quantitative RT-PCR expression analysis, functional complementation of the cad1 Bd4179 mutant with the wild-type BdCAD1 allele Brachypodium distachyon

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.195 G575A/G192D the nucleotide substitution G575A occurs in BdCAD1 of the Bd4179 line, and consequently induces the G192D change in the highly conserved glycine-rich NADPH binding site GLGGVG Brachypodium distachyon
1.1.1.195 additional information screening of a chemically induced population of Brachypodium mutants (Bd21-3 background) for red culm coloration and identification of two mutants, Bd4179 and Bd7591, with mutations in the BdCAD1 gene. The amount of sinapic acid ester-linked to cell walls is measured for in the lignin-related CAD grass mutants. Functional complementation of the Bd4179 mutant with the wild-type BdCAD1 allele restores the wild-type phenotype and lignification. The Bdcad1 alleles are responsible for the reddish-brown phenotype Brachypodium distachyon

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Brachypodium distachyon
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-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 internode
-
 Brachypodium distachyon
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1.1.1.195 leaf sheaths and leaf blades Brachypodium distachyon
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1.1.1.195 additional information quantitative RT-PCR expression analysis, in the Bd4179 mutant, only internodes and peduncles show detectable activity Brachypodium distachyon
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1.1.1.195 node
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 Brachypodium distachyon
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1.1.1.195 peduncle young Brachypodium distachyon
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1.1.1.195 spikelet young Brachypodium distachyon
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Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 BdCAD1
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 Brachypodium distachyon
1.1.1.195 CAD
-
 Brachypodium distachyon
1.1.1.195 cinnamyl alcohol dehydrogenase
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 Brachypodium distachyon
1.1.1.195 cinnamyl alcohol dehydrogenase 1
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 Brachypodium distachyon

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADPH highly conserved glycine-rich NADPH binding site GLGGVG Brachypodium distachyon

General Information

EC Number General Information Comment Organism
1.1.1.195 malfunction in CAD1 mutants Bd4179 and Bd7591, the mature stems displaye reduced CAD activity and lower lignin content. Their lignins are enriched in 8-O-4- and 4-O-5-coupled sinapaldehyde units, as well as resistant inter-unit bonds and free phenolic groups. By contrast, there is no increase in coniferaldehyde end groups. Saccharification assays reveal that Bd4179 and Bd7591 lines are more susceptible to enzymatic hydrolysis than wild-type plants. The Bdcad1 alleles are responsible for the reddish-brown phenotype, overview. Wild-type BdCAD1 rescues the altered lignin profile of Arabidopsis and Brachypodium CAD mutants. Saccharification yields are improved in Bdcad1 mutant lines but biomass yield is not compromised Brachypodium distachyon
1.1.1.195 physiological function involvement of BdCAD1 in lignification. Wild-type BdCAD1 rescues the altered lignin profile of Arabidopsis and Brachypodium CAD mutants Brachypodium distachyon