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Literature summary extracted from

  • Chen, S.; Glawischnig, E.; Jörgensen, K.; Naur, P.; Jörgensen, B.; Olsen, C.E.; Hansen, C.H.; Rasmussen, H.; Pickett, J.A.; Halkier, B.A.
    CYP79F1 and CYP79F2 have distinct functions in the biosynthesis of aliphatic glucosinolates in Arabidopsis (2003), Plant J., 33, 923-937.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.42 expression in Escherichia coli Arabidopsis thaliana
1.14.14.42 expression in Saccharomyces cerevisiae Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.42 0.026
-
 L-hexahomomethionine pH 7.5, 25°C Arabidopsis thaliana
1.14.14.42 0.034
-
 L-dihomomethionine pH 7.5, 25°C Arabidopsis thaliana
1.14.14.42 0.037
-
 L-trihomomethionine pH 7.5, 25°C Arabidopsis thaliana
1.14.14.42 0.074
-
 L-hexahomomethionine pH 7.5, 25°C Arabidopsis thaliana
1.14.14.42 0.194
-
 L-tetrahomomethionine pH 7.5, 25°C Arabidopsis thaliana
1.14.14.42 0.216
-
 L-pentahomomethionine pH 7.5, 25°C Arabidopsis thaliana
1.14.14.42 0.374
-
 L-pentahomomethionine pH 7.5, 25°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.42 62000
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.42 L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] Arabidopsis thaliana
-
 4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.42 L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] Arabidopsis thaliana
-
 4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] Arabidopsis thaliana
-
 4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.42 L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] Arabidopsis thaliana
-
 4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] Arabidopsis thaliana
-
 4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] Arabidopsis thaliana
-
 4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 additional information Arabidopsis thaliana isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine ?
-
 ?
1.14.14.42 additional information Arabidopsis thaliana isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.42 Arabidopsis thaliana Q949U1
-
-
1.14.14.42 Arabidopsis thaliana Q9FUY7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.42 cotyledon strongly expressed in cotyledons, rosette leaves, stems, and siliques Arabidopsis thaliana
-
1.14.14.42 hypocotyl strongly expressed in hypocotyl and root Arabidopsis thaliana
-
1.14.14.42 leaf strongly expressed in cotyledons, rosette leaves, stems, and siliques Arabidopsis thaliana
-
1.14.14.42 root strongly expressed in hypocotyl and root Arabidopsis thaliana
-
1.14.14.42 silique strongly expressed in cotyledons, rosette leaves, stems, and siliques Arabidopsis thaliana
-
1.14.14.42 stem strongly expressed in cotyledons, rosette leaves, stems, and siliques Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.42 L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.42 L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
 ?
1.14.14.42 L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Arabidopsis thaliana 4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase] overall reaction ?
1.14.14.42 additional information isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine Arabidopsis thaliana ?
-
 ?
1.14.14.42 additional information isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.42 ? x * 62000, calculated and SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.14.42 CYP79F1
-
 Arabidopsis thaliana
1.14.14.42 CYP79F2
-
 Arabidopsis thaliana
1.14.14.42 hexahomomethionine N-hydroxylase
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.42 NADPH
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.14.42 physiological function a transposon-tagged CYP79F1 knockout mutant completely lacks short-chain aliphatic glucosinolates, but has an increased level of long-chain aliphatic glucosinolates, especially in leaves and seeds Arabidopsis thaliana
1.14.14.42 physiological function the level of long-chain aliphatic glucosinolates in a transposon-tagged CYP79F2 knockout mutant is substantially reduced, whereas the level of short-chain aliphatic glucosinolates is not affected Arabidopsis thaliana