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Literature summary extracted from

  • de Kraker, J.W.; Gershenzon, J.
    From amino acid to glucosinolate biosynthesis: protein sequence changes in the evolution of methylthioalkylmalate synthase in Arabidopsis (2011), Plant Cell, 23, 38-53.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.17 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.17 53000
-
-
 Arabidopsis thaliana
2.3.3.17 53200
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.17 an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O Arabidopsis thaliana
-
 a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.17 Arabidopsis thaliana Q9FG67 ecotype Columbia-0
-
2.3.3.17 Arabidopsis thaliana Q9FN52 ecotype Columbia-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.17 Ni-NTA agarose column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.17 4-methylthio-2-oxobutyrate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(2'-methylthioethyl)malate + CoA
-
 ?
2.3.3.17 5-methylthio-2-oxopentanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana ?
-
 ?
2.3.3.17 6-methylthio-2-oxohexanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana 2-(4-methylthiobutyl)malate + CoA
-
 ?
2.3.3.17 an omega-(methylthio)-2-oxoalkanoate + acetyl-CoA + H2O
-
 Arabidopsis thaliana a 2-[omega-(methylthio)alkyl]malate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.17 monomer 1 * 53000, isoform MAM3, calculated from amino acid sequence Arabidopsis thaliana
2.3.3.17 monomer 1 * 53200, isoform MAM1, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.3.17 MAM1 isoform Arabidopsis thaliana
2.3.3.17 MAM3 isoform Arabidopsis thaliana