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Literature summary extracted from

  • Bak, S.; Tax, F.E.; Feldmann, K.A.; Galbraith, D.W.; Feyereisen, R.
    CYP83B1, a cytochrome P450 at the metabolic branch point in auxin and indole glucosinolate biosynthesis in Arabidopsis (2001), Plant Cell, 13, 101-111.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.45 expression in Saccharomyces cerevisiae Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.45 tryptamine
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.45 0.003
-
 indole-3-acetaldoxime pH 7.6, 28°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.45 indole-3-acetaldoxime + [reduced NADPH-hemoprotein reductase] + O2 Arabidopsis thaliana
-
 1-aci-nitro-2-indolyl-ethane + [oxidized NADPH-hemoprotein reductase] + 2 H2O the aci-nitro compound formed reacts non-enzymatically with thiol compounds to produce an N-alkyl-thiohydroximate adduct, the committed precursor of glucosinolates ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.45 Arabidopsis thaliana O65782
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.45 indole-3-acetaldoxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 1-aci-nitro-2-indolyl-ethane + [oxidized NADPH-hemoprotein reductase] + 2 H2O the aci-nitro compound formed reacts non-enzymatically with thiol compounds to produce an N-alkyl-thiohydroximate adduct, the committed precursor of glucosinolates ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.45 0.88
-
 indole-3-acetaldoxime pH 7.6, 28°C Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.14.45 physiological function a T-DNA insertion in the CYP83B1 gene leads to plants with a phenotype that suggests severe auxin overproduction. Mutant seedlings are characterized by increased hypocotyl length, epinastic cotyledons, exfoliation of the hypocotyl, adventitious root formation from the hypocotyl, enhanced secondary root and root hair formation, and eventually callus formation and increasing disintegration of the seedling. Mutant seedlings contain reduced levels of indole glucosinolates. CYP83B1 overexpression leads to loss of apical dominance typical of auxin deficit Arabidopsis thaliana