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Literature summary extracted from

  • Miyagawa, N.; Miyahara, T.; Okamoto, M.; Hirose, Y.; Sakaguchi, K.; Hatano, S.; Ozeki, Y.
    Dihydroflavonol 4-reductase activity is associated with the intensity of flower colors in Delphinium (2015), Plant Biotechnol., 32, 249-255.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 gene DcDFR, genotyping, quantitative enzyme expression analysis Delphinium cardinale
1.1.1.219 gene DgDFR, genotyping, quantitative enzyme expression analysis Delphinium grandiflorum
1.1.1.219 gene DnDFR, genotyping, quantitative enzyme expression analysis Delphinium nudicaule

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.219 0.0005
-
 dihydromyricetin pH 7.0, 30°C Delphinium grandiflorum
1.1.1.219 0.002
-
 dihydroquercetin pH 7.0, 30°C Delphinium grandiflorum
1.1.1.219 0.0061
-
 dihydrokaempferol pH 7.0, 30°C Delphinium grandiflorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 dihydrokaempferol + NADPH + H+ Delphinium grandiflorum
-
 leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+ Delphinium nudicaule
-
 leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+ Delphinium cardinale
-
 leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+ Delphinium grandiflorum
-
 leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+ Delphinium nudicaule
-
 leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+ Delphinium cardinale
-
 leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+ Delphinium grandiflorum
-
 cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+ Delphinium nudicaule
-
 cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+ Delphinium cardinale
-
 cis-3,4-leucocyanidin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Delphinium cardinale A0A0C6ENR6 several cultivars
-
1.1.1.219 Delphinium grandiflorum A0A0C6EUC4 var. chinense, several cultivars
-
1.1.1.219 Delphinium nudicaule A0A0C6E754 several cultivars
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 flower quantitative enzyme expression analysis in flowers from different cultivars, overview Delphinium grandiflorum
-
1.1.1.219 flower quantitative enzyme expression analysis in flowers from different cultivars, overview Delphinium nudicaule
-
1.1.1.219 flower quantitative enzyme expression analysis in flowers from different cultivars, overview Delphinium cardinale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Delphinium grandiflorum leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Delphinium nudicaule leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+
-
 Delphinium cardinale leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+ best substrate Delphinium grandiflorum leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydrokaempferol + NADPH + H+ best substrate Delphinium nudicaule leucopelargonidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Delphinium grandiflorum leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Delphinium nudicaule leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+
-
 Delphinium cardinale leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+ 14% of the activity with dihydrokaempferol Delphinium grandiflorum leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydromyricetin + NADPH + H+ 23% of the activity with dihydrokaempferol Delphinium nudicaule leucodelphinidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Delphinium grandiflorum cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Delphinium nudicaule cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Delphinium cardinale cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+ 11% of the activity with dihydrokaempferol Delphinium nudicaule cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+ 14% of the activity with dihydrokaempferol Delphinium grandiflorum cis-3,4-leucocyanidin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DcDFR
-
 Delphinium cardinale
1.1.1.219 DFR
-
 Delphinium grandiflorum
1.1.1.219 DFR
-
 Delphinium nudicaule
1.1.1.219 DFR
-
 Delphinium cardinale
1.1.1.219 DgDFR
-
 Delphinium grandiflorum
1.1.1.219 DnDFR
-
 Delphinium nudicaule

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 30
-
 assay at Delphinium grandiflorum
1.1.1.219 30
-
 assay at Delphinium nudicaule
1.1.1.219 30
-
 assay at Delphinium cardinale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.219 7
-
 assay at Delphinium grandiflorum
1.1.1.219 7
-
 assay at Delphinium nudicaule
1.1.1.219 7
-
 assay at Delphinium cardinale

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Delphinium grandiflorum
1.1.1.219 NADPH
-
 Delphinium nudicaule
1.1.1.219 NADPH
-
 Delphinium cardinale

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.219 0.0016
-
 dihydromyricetin pH 7.0, 30°C Delphinium cardinale
1.1.1.219 0.0023
-
 dihydroquercetin pH 7.0, 30°C Delphinium cardinale
1.1.1.219 0.006
-
 dihydrokaempferol pH 7.0, 30°C Delphinium cardinale
1.1.1.219 0.0061
-
 dihydrokaempferol pH 7.0, 30°C Delphinium nudicaule