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Literature summary extracted from

  • Ragamustari, S.; Shiraiwa, N.; Hattori, T.; Nakatsubo, T.; Suzuki, S.; Umezawa, T.
    Characterization of three cinnamyl alcohol dehydrogenases from Carthamus tinctorius (2013), Plant Biotechnol., 30, 315-326.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 gene ctcad1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis Carthamus tinctorius
1.1.1.195 gene ctcad2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis Carthamus tinctorius
1.1.1.195 quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius Carthamus tinctorius

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.195 coniferyl aldehyde competitve, a stronger inhibitor than sinapyl aldehyde; competitve, a stronger inhibitor than sinapyl aldehyde; competitve, a stronger inhibitor than sinapyl aldehyde Carthamus tinctorius
1.1.1.195 sinapyl aldehyde competitve, a weaker inhibitor than coniferyl aldehyde; competitve, a weaker inhibitor than coniferyl aldehyde; competitve, a weaker inhibitor than coniferyl aldehyde Carthamus tinctorius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.0058
-
 sinapyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.0068
-
 coniferyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.024
-
 coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.026
-
 sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.032
-
 coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.055
-
 sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.195 Zn2+ required, Zn1 binding sequence is located at amino acid residues 68-82, and Zn2 binding sequence is located at amino acid residues 88-113 Carthamus tinctorius
1.1.1.195 Zn2+ required, Zn1 binding sequence is located at amino acid residues 69-83, and Zn2 binding sequence is located at amino acid residues 89-114 Carthamus tinctorius
1.1.1.195 Zn2+ required, Zn1 binding sequence is located at amino acid residues 71-85, and Zn2 binding sequence is located at amino acid residues 91-116 Carthamus tinctorius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.195 cinnamaldehyde + NADPH + H+ Carthamus tinctorius
-
 cinnamyl alcohol + NADP+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH + H+ Carthamus tinctorius
-
 coniferyl alcohol + NADP+
-
 r
1.1.1.195 sinapyl aldehyde + NADPH + H+ Carthamus tinctorius
-
 sinapyl alcohol + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Carthamus tinctorius U3TDQ6 CAD3
-
1.1.1.195 Carthamus tinctorius U3TH11 CAD2
-
1.1.1.195 Carthamus tinctorius U3TI46 CAD1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 seed quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius Carthamus tinctorius
-
1.1.1.195 stem
-
 Carthamus tinctorius
-
1.1.1.195 stem low expression of CAD3 in stem Carthamus tinctorius
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 cinnamaldehyde + NADPH + H+
-
 Carthamus tinctorius cinnamyl alcohol + NADP+
-
 r
1.1.1.195 coniferyl aldehyde + NADPH + H+
-
 Carthamus tinctorius coniferyl alcohol + NADP+
-
 r
1.1.1.195 sinapyl aldehyde + NADPH + H+
-
 Carthamus tinctorius sinapyl alcohol + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD
-
 Carthamus tinctorius
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Carthamus tinctorius
1.1.1.195 CtCAD1
-
 Carthamus tinctorius
1.1.1.195 CtCAD2
-
 Carthamus tinctorius
1.1.1.195 CtCAD3
-
 Carthamus tinctorius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.195 26
-
 assay at Carthamus tinctorius
1.1.1.195 30
-
 assay at Carthamus tinctorius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.195 0.00098
-
 coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.00135
-
 sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.0053
-
 sinapyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.0121
-
 coniferyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.032
-
 coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.041
-
 sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.195 6.5 7.5 assay at Carthamus tinctorius

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADP+
-
 Carthamus tinctorius
1.1.1.195 NADPH the NADPH binding sequence is located at amino acid residues 188-193 Carthamus tinctorius
1.1.1.195 NADPH the NADPH binding sequence is located at amino acid residues 189-194 Carthamus tinctorius
1.1.1.195 NADPH the NADPH binding sequence is located at amino acid residues 191-196 Carthamus tinctorius

General Information

EC Number General Information Comment Organism
1.1.1.195 additional information enzyme structure modelling and molecular docking, substrate binding pocket structure, overview Carthamus tinctorius

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.195 0.0245
-
 sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 0.0306
-
 coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 1.32
-
 coniferyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 1.58
-
 sinapyl aldehyde pH 6.5-7.5, 30°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 5.39
-
 sinapyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius
1.1.1.195 10.62
-
 coniferyl aldehyde pH 6.5-7.5, 26°C, recombinant enzyme Carthamus tinctorius