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Literature summary extracted from

  • Qiang, W.; Xia, K.; Zhang, Q.; Zeng, J.; Huang, Y.; Yang, C.; Chen, M.; Liu, X.; Lan, X.; Liao, Z.
    Functional characterisation of a tropine-forming reductase gene from Brugmansia arborea, a woody plant species producing tropane alkaloids (2016), Phytochemistry, 127, 12-22.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.206 gene TRI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, functional recombinant overexpression of His-tagged enzyme in Escherichia coli strain Rosetta Brugmansia arborea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.206 additional information
-
 additional information Michaelis-Menten kinetics Brugmansia arborea
1.1.1.206 additional information
-
 additional information Michaelis–Menten kinetics Datura stramonium
1.1.1.206 0.34
-
 Tropine pH 9.6, 22°C Datura stramonium
1.1.1.206 0.46
-
 4-methylcyclohexanone pH 6.4, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 0.56
-
 Tropine pH 9.6, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 2.65
-
 tropinone pH 6.4, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 4.18
-
 tropinone pH 6.4, 22°C Datura stramonium
1.1.1.206 5.17
-
 3-quinuclidinone pH 9.6, 22°C, recombinant His-tagged enzyme Brugmansia arborea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.206 29000
-
-
 Brugmansia arborea
1.1.1.206 29700
-
-
 Brugmansia arborea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.206 tropinone + NADPH + H+ Datura stramonium
-
 tropine + NADP+
-
 r
1.1.1.206 tropinone + NADPH + H+ Brugmansia arborea
-
 tropine + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.206 Brugmansia arborea A0A088MI02 formerly Datura arborea
-
1.1.1.206 Datura stramonium P50162
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.206 recombinant His-tagged enzyme from Escherichia coli strain Rosetta by nickel affinity chromatography Brugmansia arborea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.206 leaf
-
 Brugmansia arborea
-
1.1.1.206 additional information enzyme BaTRI is expressed in all examined organs but is most abundant in secondary roots Brugmansia arborea
-
1.1.1.206 root
-
 Brugmansia arborea
-
1.1.1.206 stem
-
 Brugmansia arborea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.206 3-quinuclidinone + NADPH + H+
-
 Datura stramonium 1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
 r
1.1.1.206 3-quinuclidinone + NADPH + H+
-
 Brugmansia arborea 1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
 r
1.1.1.206 4-methylcyclohexanone + NADPH + H+
-
 Datura stramonium 4-methylcyclohexanol + NADP+
-
 r
1.1.1.206 4-methylcyclohexanone + NADPH + H+
-
 Brugmansia arborea 4-methylcyclohexanol + NADP+
-
 r
1.1.1.206 additional information at pH 6.4, the non-ionizable substrate 4-methylcyclohexanone is uncharged, whereas the nitrogen-containing substrates tropinone and 3-quinuclidinone are positively charged. Enzyme BaTRI exhibits significantly higher apparent affinity for 4-methylcyclohexanone than tropinone and 3-quinuclidinone, of which only the deprotonated fractions can be bound by BaTRI Brugmansia arborea ?
-
 ?
1.1.1.206 tropine + NADP+
-
 Datura stramonium tropinone + NADPH + H+
-
 r
1.1.1.206 tropine + NADP+
-
 Brugmansia arborea tropinone + NADPH + H+
-
 r
1.1.1.206 tropinone + NADPH + H+
-
 Datura stramonium tropine + NADP+
-
 r
1.1.1.206 tropinone + NADPH + H+
-
 Brugmansia arborea tropine + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.206 ? x * 29000, recombinant enzyme, SDS-PAGE, x * 29700, about, sequence calculation Brugmansia arborea
1.1.1.206 More enzyme three-dimensional structure molecular modeling, overview Brugmansia arborea

Synonyms

EC Number Synonyms Comment Organism
1.1.1.206 BaTRI
-
 Brugmansia arborea
1.1.1.206 DsTRI
-
 Datura stramonium
1.1.1.206 TRI
-
 Datura stramonium
1.1.1.206 TRI
-
 Brugmansia arborea
1.1.1.206 tropine-forming reductase
-
 Datura stramonium
1.1.1.206 tropine-forming reductase
-
 Brugmansia arborea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.206 0.54
-
 3-quinuclidinone pH 9.6, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 2.4
-
 tropinone pH 6.4, 22°C Datura stramonium
1.1.1.206 2.93
-
 tropinone pH 6.4, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 3.3
-
 Tropine pH 9.6, 22°C Datura stramonium
1.1.1.206 4.67
-
 4-methylcyclohexanone pH 6.4, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 5.69
-
 Tropine pH 9.6, 22°C, recombinant His-tagged enzyme Brugmansia arborea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.206 6.8 8 tropinone reduction reaction Datura stramonium
1.1.1.206 6.8 8 tropinone reduction reaction Brugmansia arborea
1.1.1.206 9.6
-
 tropine oxidation reaction Brugmansia arborea
1.1.1.206 9.9
-
 tropine oxidation reaction Datura stramonium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.206 NADP+
-
 Datura stramonium
1.1.1.206 NADP+
-
 Brugmansia arborea
1.1.1.206 NADPH
-
 Datura stramonium
1.1.1.206 NADPH
-
 Brugmansia arborea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.206 Datura stramonium sequence calculation
-
 5.79
1.1.1.206 Brugmansia arborea sequence calculation
-
 5.79

General Information

EC Number General Information Comment Organism
1.1.1.206 evolution enzyme TRI belongs to the family of short chain dehydrogenases/reductases (SDRs) that catalyse NAD(P)(H)-dependent redox reactions Datura stramonium
1.1.1.206 evolution enzyme TRI belongs to the family of short chain dehydrogenases/reductases (SDRs) that catalyse NAD(P)(H)-dependent redox reactions Brugmansia arborea
1.1.1.206 metabolism two tropinone reductases (TRs) constitute an important branch point in the tropanalkaloid biosynthetic pathway. Tropinone reductase I or tropine-forming reductase, EC 1.1.1.206, reduces tropinone to tropine during tropanalkaloid biosynthesis, whereas tropinone reductase II, EC 1.1.1.236, reduces tropinone to pseudotropine, diverging metabolic flux to nortropane calystegine A3. TRI activity controls metabolic flux towards hyoscyamine and downstream tropanalkaloids' biosynthesis Datura stramonium
1.1.1.206 metabolism two tropinone reductases (TRs) constitute an important branch point in the tropanalkaloid biosynthetic pathway. Tropinone reductase I or tropine-forming reductase, EC 1.1.1.206, reduces tropinone to tropine during tropanalkaloid biosynthesis, whereas tropinone reductase II, EC 1.1.1.236, reduces tropinone to pseudotropine, diverging metabolic flux to nortropane calystegine A3. TRI activity controls metabolic flux towards hyoscyamine and downstream tropanalkaloids' biosynthesis Brugmansia arborea
1.1.1.206 additional information enzyme three-dimensional structure molecular modeling, overview Brugmansia arborea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.206 0.1045
-
 3-quinuclidinone pH 9.6, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 0.574
-
 tropinone pH 6.4, 22°C Datura stramonium
1.1.1.206 1.106
-
 tropinone pH 6.4, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 9.706
-
 Tropine pH 9.6, 22°C Datura stramonium
1.1.1.206 10.15
-
 4-methylcyclohexanone pH 6.4, 22°C, recombinant His-tagged enzyme Brugmansia arborea
1.1.1.206 10.16
-
 Tropine pH 9.6, 22°C, recombinant His-tagged enzyme Brugmansia arborea