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Literature summary extracted from

  • Bonin, P.; Groisillier, A.; Raimbault, A.; Guibert, A.; Boyen, C.; Tonon, T.
    Molecular and biochemical characterization of mannitol-1-phosphate dehydrogenase from the model brown alga Ectocarpus sp. (2015), Phytochemistry, 117, 509-520.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 gene MPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis Ectocarpus siliculosus
1.1.1.17 gene MPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis, recombinant expression of His-tagged EsM1PDH1 in Escherichia coli Ectocarpus siliculosus
1.1.1.17 gene MPDH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis Ectocarpus siliculosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 additional information no inhibition by EDTA; no inhibition by EDTA; no inhibition by EDTA Ectocarpus siliculosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 additional information
-
 additional information Michaelis-Menten kinetics Ectocarpus siliculosus
1.1.1.17 0.02
-
 NAD+ recombinant enzyme, pH 9.0, 30°C Ectocarpus siliculosus
1.1.1.17 0.19
-
 D-fructose 6-phosphate recombinant enzyme, pH 7.0, 30°C Ectocarpus siliculosus
1.1.1.17 0.38
-
 D-mannitol 1-phosphate recombinant enzyme, pH 9.0, 30°C Ectocarpus siliculosus
1.1.1.17 16
-
 NADH recombinant enzyme, pH 7.0, 30°C Ectocarpus siliculosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.17 NaCl activates Ectocarpus siliculosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 42000
-
-
 Ectocarpus siliculosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Ectocarpus siliculosus
-
 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 additional information Ectocarpus siliculosus mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Ectocarpus siliculosus D7FMJ8 MPDH1
-
1.1.1.17 Ectocarpus siliculosus D7G793 MPDH3
-
1.1.1.17 Ectocarpus siliculosus D8LI09 MPDH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 recombinant His-tagged EsM1PDH1 from Escherichia coli by nickel affinity chromatography and gel filtration Ectocarpus siliculosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 0.014
-
 recombinant enzyme, D-mannitol 1-phosphate oxidation, pH 9.0, 30°C Ectocarpus siliculosus
1.1.1.17 0.016
-
 purified recombinant enzyme, D-mannitol 1-phosphate oxidation, pH 9.0, 30°C Ectocarpus siliculosus
1.1.1.17 0.026
-
 recombinant enzyme, D-fructose 6-phosphate reduction, pH 7.0, 30°C Ectocarpus siliculosus
1.1.1.17 0.046
-
 purified recombinant enzyme, D-fructose 6-phosphate reduction, pH 7.0, 30°C Ectocarpus siliculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Ectocarpus siliculosus D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 additional information mannitol is present in large amounts in brown algae, where its synthesis involved two steps: a mannitol-1-phosphate dehydrogenase (M1PDH) catalyzes a reversible reaction between fructose-6-phosphate (F6P) and mannitol-1-phosphate (M1P) (EC 1.1.1.17), and a mannitol-1-phosphatase hydrolyzes M1P to mannitol (EC 3.1.3.22) Ectocarpus siliculosus ?
-
 ?
1.1.1.17 additional information EsM1PDH1cat isozyme is specific for D-fructose 6-phosphate and D-mannitol 1-phosphate Ectocarpus siliculosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.17 ? x * 42000, recombinant His-tagged EsM1PDH1 isozyme, SDS-PAGE Ectocarpus siliculosus
1.1.1.17 More structure-based sequence comparisons, overview Ectocarpus siliculosus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 EsM1PDH1
-
 Ectocarpus siliculosus
1.1.1.17 EsM1PDH2
-
 Ectocarpus siliculosus
1.1.1.17 EsM1PDH3
-
 Ectocarpus siliculosus
1.1.1.17 M1PDH
-
 Ectocarpus siliculosus
1.1.1.17 mannitol-1-phosphate dehydrogenase
-
 Ectocarpus siliculosus
1.1.1.17 MPDH1
-
 Ectocarpus siliculosus
1.1.1.17 MPDH2
-
 Ectocarpus siliculosus
1.1.1.17 MPDH3
-
 Ectocarpus siliculosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 30
-
-
 Ectocarpus siliculosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.17 5 50 35% of maximum activity at 5-10°C, less than 5% at 50°C Ectocarpus siliculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 7
-
 D-fructose 6-phosphate reduction Ectocarpus siliculosus
1.1.1.17 9
-
 D-mannitol 1-phosphate oxidation Ectocarpus siliculosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 additional information isozyme EsM1PDH1 highly prefers NADH/NAD+ to NADP+/NADPH Ectocarpus siliculosus
1.1.1.17 NAD+
-
 Ectocarpus siliculosus
1.1.1.17 NADH
-
 Ectocarpus siliculosus

General Information

EC Number General Information Comment Organism
1.1.1.17 evolution sequence analysis suggests that algal and apicomplexa M1PDHs represent a distinct type of mannitol-1-phosphate dehydrogenase in the PSLDR family Ectocarpus siliculosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.17 29
-
 D-mannitol 1-phosphate recombinant enzyme, pH 9.0, 30°C Ectocarpus siliculosus
1.1.1.17 170
-
 D-fructose 6-phosphate recombinant enzyme, pH 7.0, 30°C Ectocarpus siliculosus