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Literature summary extracted from

  • Chauleau, M.; Jacewicz, A.; Shuman, S.
    DNA3pp5G de-capping activity of aprataxin: effect of cap nucleoside analogs and structural basis for guanosine recognition (2015), Nucleic Acids Res., 43, 6075-6083.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.70 crystal structure in complex with GMP, to 1.5 A resolution. GMP binds at the same position and in the same anti nucleoside conformation as AMP, and aprataxin makes more extensive nucleobase contacts with guanine than with adenine, via a hydrogen bonding network to the guanine O6, N1, N2 base edge Schizosaccharomyces pombe
3.6.1.71 crystal structure in complex with GMP, to 1.5 A resolution. GMP binds at the same position and in the same anti nucleoside conformation as AMP, and aprataxin makes more extensive nucleobase contacts with guanine than with adenine, via a hydrogen bonding network to the guanine O6, N1, N2 base edge Schizosaccharomyces pombe
3.6.1.72 crystal structure in complex with GMP, to 1.5 A resolution. GMP binds at the same position and in the same anti nucleoside conformation as AMP, and aprataxin makes more extensive nucleobase contacts with guanine than with adenine, via a hydrogen bonding network to the guanine O6, N1, N2 base edge Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.72 D63A mutation impairs DNAppG decapping Schizosaccharomyces pombe
3.6.1.72 His147A mutation abolishes DNAppG decapping activity Schizosaccharomyces pombe
3.6.1.72 His149A mutation abolishes DNAppG decapping activity Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.70 Schizosaccharomyces pombe O74859
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3.6.1.70 Schizosaccharomyces pombe ATCC 24843 O74859
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-
3.6.1.71 Schizosaccharomyces pombe O74859
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3.6.1.71 Schizosaccharomyces pombe ATCC 24843 O74859
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-
3.6.1.72 Schizosaccharomyces pombe O74859
-
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3.6.1.72 Schizosaccharomyces pombe ATCC 24843 O74859
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.70 additional information aprataxin additionally is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP, reaction of EC 3.1.12.2. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap Schizosaccharomyces pombe ?
-
 ?
3.6.1.70 additional information aprataxin additionally is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP, reaction of EC 3.1.12.2. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap Schizosaccharomyces pombe ATCC 24843 ?
-
 ?
3.6.1.71 additional information aprataxin additionally is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP, reaction of EC 3.1.12.2. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap Schizosaccharomyces pombe ?
-
 ?
3.6.1.71 additional information aprataxin additionally is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP, reaction of EC 3.1.12.2. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap Schizosaccharomyces pombe ATCC 24843 ?
-
 ?
3.6.1.72 additional information aprataxin is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap. The enzyme also possesses the activity of EC 3.1.11.7, adenosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.1.11.8, guanosine-5'-diphospho-5'-[DNA] diphosphatase Schizosaccharomyces pombe ?
-
 ?
3.6.1.72 [DNA]-3'-diphospho-5'-(6-O-methylguanosine) + H2O
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 Schizosaccharomyces pombe [DNA]-3'-phosphate + 6-O-methyl-GMP
-
 ?
3.6.1.72 [DNA]-3'-diphospho-5'-(6-O-methylguanosine) + H2O
-
 Schizosaccharomyces pombe ATCC 24843 [DNA]-3'-phosphate + 6-O-methyl-GMP
-
 ?
3.6.1.72 [DNA]-3'-diphospho-5'-guanosine + H2O
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 Schizosaccharomyces pombe [DNA]-3'-phosphate + GMP
-
 ?
3.6.1.72 [DNA]-3'-diphospho-5'-guanosine + H2O
-
 Schizosaccharomyces pombe ATCC 24843 [DNA]-3'-phosphate + GMP
-
 ?
3.6.1.72 [DNA]-3'-diphospho-5'-inosine + H2O
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 Schizosaccharomyces pombe [DNA]-3'-phosphate + IMP
-
 ?
3.6.1.72 [DNA]-3'-diphospho-5'-inosine + H2O
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 Schizosaccharomyces pombe ATCC 24843 [DNA]-3'-phosphate + IMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.70 aprataxin
-
 Schizosaccharomyces pombe
3.6.1.70 HNT3
-
 Schizosaccharomyces pombe
3.6.1.71 aprataxin
-
 Schizosaccharomyces pombe
3.6.1.71 HNT3
-
 Schizosaccharomyces pombe
3.6.1.72 aprataxin
-
 Schizosaccharomyces pombe
3.6.1.72 HNT3
-
 Schizosaccharomyces pombe